1. Bacillus Calmette-Guerin vaccination at birth and in vitro cytokine responses to non-specific stimulation. A randomized clinical trial
- Author
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Christine Stabell Benn, Peter Aaby, Rob J.W. Arts, Susanne Dam Nielsen, Jesper Kjaergaard, Lone Graff Stensballe, Mihai G. Netea, Thomas Nørrelykke Nissen, Andreas Andersen, Nina Marie Birk, Poul-Erik Kofoed, Lisbeth Marianne Thøstesen, Dorthe Lisbeth Jeppesen, Morten Ruhwald, Bastiaan A. Blok, Ole Pryds, and Thomas Hoffmann
- Subjects
Male ,0301 basic medicine ,Cytokine response ,Mycobacterium bovis/immunology ,Candida albicans/immunology ,medicine.medical_treatment ,lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] ,Stimulation ,medicine.disease_cause ,law.invention ,0302 clinical medicine ,Medical microbiology ,Randomized controlled trial ,law ,Candida albicans ,030212 general & internal medicine ,Streptococcus pneumoniae/immunology ,Vaccines ,Vaccination ,General Medicine ,Mycobacterium bovis ,3. Good health ,Streptococcus pneumoniae ,Infectious Diseases ,Cytokine ,BCG Vaccine ,Cytokines ,Female ,Randomized clinical trial ,Infants ,Microbiology (medical) ,medicine.medical_specialty ,Randomization ,Trained immunity ,Cytokines/blood ,complex mixtures ,03 medical and health sciences ,All institutes and research themes of the Radboud University Medical Center ,Antigen ,BCG Vaccine/administration & dosage ,Bacillus Calmette-Guérin ,Journal Article ,Escherichia coli ,medicine ,Humans ,Heterolougus immunity ,Escherichia coli/immunology ,business.industry ,Infant, Newborn ,030104 developmental biology ,Immunology ,business - Abstract
Several studies have shown increased in vitro cytokine responses to non-related pathogens after Bacillus Calmette-Guérin (BCG) vaccination. A total of 158 infants (80 BCG administered within 7 days of birth; 78 controls) were bled 4 days post-randomization, and at age 3 and 13 months. Geometric mean concentrations of IL-1β, TNF-α, IL-6 (24 h stimulation) and IFN-γ, IL-10, IL-17, IL-22 (96 h stimulation) in response to in vitro stimulation with RPMI, LPS, PHA, Escherichia coli, Streptococcus pneumoniae, Candida albicans and BCG were compared among BCG vaccinated children and controls. BCG vaccination did not affect in vitro cytokine production, except IFN-γ and IL-22 response to BCG. Stratifying for 'age at randomization' we found a potentiating effect of BCG on cytokine production (TNF-α, IL-6, IL-10) in the 4 days post randomization stimulations, among children who were vaccinated at age 2-7 days versus age 0-1 days. BCG vaccination did not potentiate cytokine production to non-BCG antigens. At 4 days post randomization, BCG was associated with higher cytokine production in the later randomized children.
- Published
- 2018