1. Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota.
- Author
-
Albani D, Giorgetti L, Pitto L, Luo M, Cantoni RM, Erra Pujada M, Rotino GL, and Cella R
- Subjects
- Cell Line, Cell Proliferation, Daucus carota genetics, Daucus carota growth & development, Gene Expression Regulation, Enzymologic genetics, In Situ Hybridization, Promoter Regions, Genetic genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Tetrahydrofolate Dehydrogenase metabolism, Thymidylate Synthase metabolism, Daucus carota enzymology, Gene Expression Regulation, Plant genetics, Tetrahydrofolate Dehydrogenase genetics, Thymidylate Synthase genetics
- Abstract
The pattern of expression of a carrot dhfr-ts gene was evaluated in different plant organs, in somatic embryos, and in hypocotyl explants induced to dedifferentiate in vitro by the addition of the synthetic auxin 2,4 dichorophenoxyacetic acid. The promoter of this gene was also placed upstream of a uidA (GUS) reporter gene and, using biolistic and protoplasts transient expression assays, was shown to drive a particularly high level of expression in actively growing suspension cells. The results from these expression analyses combined with the presence of putative cell cycle-related cis-acting elements in the dhfr-ts promoter, strongly point to a cell division-dependent expression of this gene.
- Published
- 2005