1. Antibacterial active compounds from Hypericum ascyron L. induce bacterial cell death through apoptosis pathway.
- Author
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Li XM, Luo XG, Si CL, Wang N, Zhou H, He JF, and Zhang TC
- Subjects
- Anti-Bacterial Agents chemistry, Anti-Bacterial Agents isolation & purification, Dose-Response Relationship, Drug, Enterobacter cloacae cytology, Enterobacter cloacae drug effects, Escherichia coli cytology, Escherichia coli drug effects, Klebsiella pneumoniae cytology, Klebsiella pneumoniae drug effects, Microbial Sensitivity Tests, Micrococcus luteus cytology, Micrococcus luteus drug effects, Molecular Structure, Plant Extracts chemistry, Plant Extracts isolation & purification, Staphylococcus aureus cytology, Staphylococcus aureus drug effects, Structure-Activity Relationship, Anti-Bacterial Agents pharmacology, Apoptosis drug effects, Hypericum chemistry, Microbial Viability drug effects, Plant Extracts pharmacology
- Abstract
Hypericum ascyron L. has been used as a traditional medicine for the treatment of wounds, swelling, headache, nausea and abscesses in China for thousands of years. However, modern pharmacological studies are still necessary to provide a scientific basis to substantiate their traditional use. In this study, the mechanism underlying the antimicrobial effect of the antibacterial activity compounds from H. ascyron L. was investigated. Bioguided fractionation of the extract from H. ascyron L. afforded antibacterial activity fraction 8. The results of cup plate analysis and MTT assay showed that the MIC and MBC of fraction 8 is 5 mg/mL. Furthermore, using Annexin V-FITC/PI, TUNEL labeling and DNA gel electrophoresis, we found that cell death with apoptosis features similar to those in eucaryon could be induced in bacteria strains after exposure to the antibacterial activity compounds from H. ascyron L. at moderate concentration. In addition, we further found fraction 8 could disrupt the cell membrane potential indicate that fraction 8 exerts pro-apoptotic effects through a membrane-mediated apoptosis pathway. Finally, quercetin and kaempferol 3-O-β-(2″-acetyl)-galactopyranoside, were identified from fraction 8 by means of Mass spectrometry and Nuclear magnetic resonance. To our best knowledge, this study is the first to show that Kaempferol 3-O-β-(2″-acetyl)-galactopyranoside coupled with quercetin had significant antibacterial activity via apoptosis pathway, and it is also the first report that Kaempferol 3-O-β-(2″-acetyl)-galactopyranoside was found in clusiacea. Our data might provide a rational base for the use of H. ascyron L. in clinical, and throw light on the development of novel antibacterial drugs., (Copyright © 2015 Elsevier Masson SAS. All rights reserved.)
- Published
- 2015
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