1. Chromatographic separation of piracetam and its metabolite in a mixture of microsomal preparations, followed by an MS/MS analysis.
- Author
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Sahu, Kapendra, Siddiqui, Anees A., Shaharyar, Mohammad, Ahmad, Niyaz, Anwar, Mohammad, and Ahmad, Farhan J.
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CHROMATOGRAPHIC analysis , *PIRACETAM , *DRUG metabolism , *SEPARATION (Technology) , *HIGH performance liquid chromatography , *CYTOCHROME P-450 , *PHARMACOKINETICS - Abstract
Abstract: A rapid bioanalytical method was evaluated for the simultaneous determination of piracetam and its metabolite (M1) in human microsomal preparations by fast ultra-performance liquid chromatography/tandem mass spectrometry (UPLC–MS/MS). In addition, a validated method of M1 in rat plasma was developed and successfully applied on pharmacokinetic studies. The present study was carried out to determine the metabolic pathways of piracetam for phase I metabolism and used cytochrome P450 isoforms responsible for the piracetam metabolism in human liver microsomes (HLMs). While additional potential metabolites of piracetam were suggested by computer-modeling. The resulting 2-(2-oxopyrrolidin-1-yl) acetic acid was the sole metabolite detected after the microsomal treatment. The amide hydrolysis mainly underwent to form a metabolite i.e., 2-(2-oxopyrrolidin-1-yl) acetic acid (M1). [Copyright &y& Elsevier]
- Published
- 2013
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