1. Nerve injury-induced calcium channel alpha-2-delta-1 protein dysregulation leads to increased pre-synaptic excitatory input into deep dorsal horn neurons and neuropathic allodynia.
- Author
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Zhou C and Luo ZD
- Subjects
- Animals, Disease Models, Animal, Male, Mice, Mice, Transgenic, Up-Regulation, Calcium Channels metabolism, Excitatory Postsynaptic Potentials physiology, Hyperalgesia etiology, Hyperalgesia metabolism, Hyperalgesia physiopathology, Neuralgia etiology, Neuralgia metabolism, Neuralgia physiopathology, Peripheral Nerve Injuries complications, Peripheral Nerve Injuries metabolism, Peripheral Nerve Injuries physiopathology, Posterior Horn Cells physiology, Spinal Nerves injuries, Touch Perception physiology
- Abstract
Background: Up-regulation of voltage-gated calcium channel α2 δ1 subunit post spinal nerve ligation (SNL) injury or in α2 δ1 -overexpressing transgenic (Tg) mice correlates with tactile allodynia, a pain state mediated mainly by Aβ sensory fibres forming synaptic connections with deep dorsal horn (DDH) neurons. It is not clear, however, whether dysregulated α2 δ1 alters DDH synaptic neurotransmission that underlies tactile allodynia development post nerve injury., Methods: Tactile allodynia was tested in the SNL and α2 δ1 Tg models. Miniature excitatory/inhibitory post-synaptic currents were recorded in DDH neurons from these animal models using whole-cell patch clamp slice recording techniques., Results: There was a significant increase in the frequency, but not amplitude, of miniature excitatory post-synaptic currents (mEPSC) in DDH neurons that correlated with tactile allodynia in SNL and α2 δ1 Tg mice. Gabapentin, an α2 δ1 ligand that is known to block tactile allodynia in these models, also normalized mEPSC frequency dose-dependently in DDH neurons from SNL and α2 δ1 Tg mice. In contrast, neither frequency nor amplitude of miniature inhibitory post-synaptic currents was altered in DDH neurons from SNL and α2 δ1 Tg mice., Conclusion: Our data suggest that α2 δ1 dysregulation is highly likely contributing to tactile allodynia through a pre-synaptic mechanism involving facilitation of excitatory synaptic neurotransmission in DDH of spinal cord., (© 2015 European Pain Federation - EFIC®)
- Published
- 2015
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