Search

Your search keyword '"Griese, M"' showing total 6 results
Start Over Author "Griese, M" Journal european journal of pediatrics
6 results on '"Griese, M"'

5. Beta-adrenoceptor density and resolution of high and low affinity state on B- and T-cells in asthmatic and non-asthmatic children.

Author

Körholz, D, Seeger, K, Griese, M, Wahn, V, Reifenhäuser, A, and Reinhardt, D

Abstract

Beta-adrenoceptor binding in lymphocytes of asthmatic and non-asthmatic children and healthy adult volunteers was investigated with the radioligand 125-iodocyanopindolol (ICYP). Binding studies were performed with 4 to 5 different concentrations of ICYP. Receptor density and affinity were calculated by Scatchard plots. Resolution of beta-adrenoceptors into those of high and low affinity state was obtained from inhibition curves with salbutamol using Hofstee plots. Receptor density in B-cell enriched fractions was two to three-fold higher than in T-cells for all patients and volunteers studied (P less than 0.025). No difference in beta-adrenoceptor density on B and T-cells occurred neither in age-matched asthmatic and non-asthmatic children nor in adult volunteers. The affinity of beta-adrenoceptors did not differ for B and T-cells nor for the patients or volunteers studied. However, when two distinct binding states for beta-adrenoceptor agonists were obtained using salbutamol displacement curves it appeared that beta-adrenoceptors on T-cells were at a higher affinity state compared to those on B-cells in asthmatic and non-asthmatic children, as well as in adults. Since the ability of an agonist to activate adenylate cyclase correlates closely with the amount of high affinity receptor state formed in the presence of the agonist, increased intrinsic activity of the beta-adrenoceptor agonist on T-cells may be postulated. In conclusion, age-related control groups and determination of the B/T ratio are necessary for interpretation of beta-adrenoceptor changes in bronchial asthma. [ABSTRACT FROM AUTHOR]

Published
1988

6. Glucocorticoid receptors in mononuclear blood cells and their correlation to endogenous and exogenous corticoids in healthy and asthmatic children

Author

B. Roth, W. Köster, K. Lüsebring, G. Kusenbach, D. Reinhardt, and Griese M

Subjects
medicine.medical_specialty, Adolescent, Hydrocortisone, Prednisolone, Peripheral blood mononuclear cell, Dexamethasone, Excretion, Receptors, Glucocorticoid, Glucocorticoid receptor, Adrenal Cortex Hormones, Internal medicine, Humans, Medicine, Child, Receptor, Binding Sites, business.industry, Infant, Asthma, Cortisone, Endocrinology, Child, Preschool, Pediatrics, Perinatology and Child Health, Leukocytes, Mononuclear, business, Glucocorticoid, medicine.drug
Abstract

The number and affinity of glucocorticoid binding sites in peripheral mononuclear cells (MNC) of asthmatic and healthy children were determined by a whole cell (3H)dexamethasone binding assay at 37°C. Using HPLC determination, corresponding serum levels of non-protein-bound (free) cortisol, whole cortisol and cortisone as well as urine excretion of free cortisone and cortisol were assessed. The average number of binding sites (BS) per cell and the dissociation constant (KD) respectively, in atopic asthmatics (7768±666 BS/MNC resp. KD=17.2±2 nM) did not differ from the values measured in our control group (8333±691 BS/MNC resp. 25.4±4.8 nM). Within the age range 1 month-15.8 years neither age-dependent changes nor sex-related differences in the number of binding sites or the KD values could be detected. Active or currently inactive asthmatics, and patients under different antiasthmatic drug regimes, had similar binding sites on MNC. No differences in serum levels of cortisol, cortisone and free cortisol or in free cortisol and free cortisone of 24-h urine samples were found between healthy children and asthmatics. After a short course of prednisolone therapy for an acute severe asthmatic attack the number of glucocorticoid binding sites in peripheral MNC decreased to an average of 4632±421 BS/MNC, whereas the dissociation constant did not change significantly (14.5±3.6 nM). The corticod-hormone pattern in the serum, 24-h urine excretion, and the normal number and affinity of glucocorticoid receptors on peripheral MNC suggest that there is no primary, general impairment of glucocorticoid metabolism in asthmatic children. Short-term glucocorticoid administration resulted in suppression of endogenous corticoids to undetectable levels accompanied by down-regulation of glucocorticoid-receptor BS to about 55% of control levels.

Published
1988
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results