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Your search keyword '"Pantelidis P"' showing total 10 results
Start Over Author "Pantelidis P" Journal european respiratory journal
10 results on '"Pantelidis P"'

9. A molecular comparison of microbial communities in bronchiectasis and cystic fibrosis.

Author

Duff RM, Simmonds NJ, Davies JC, Wilson R, Alton EW, Pantelidis P, Cox MJ, Cookson WO, Bilton D, and Moffatt MF

Subjects
Adult, Aged, Anti-Bacterial Agents pharmacology, Bacterial Infections diagnosis, Bronchiectasis diagnosis, Cystic Fibrosis diagnosis, Humans, Inflammation, Metagenome, Middle Aged, Pseudomonas aeruginosa genetics, Pseudomonas aeruginosa isolation & purification, RNA, Ribosomal, 16S genetics, Sputum microbiology, Bacterial Infections microbiology, Bronchiectasis microbiology, Cystic Fibrosis microbiology
Published
2013
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10. Single-cell analysis: a novel approach to tumour necrosis factor-alpha synthesis and secretion in sarcoidosis.

Author

Pantelidis P, McGrath DS, Southcott AM, and du Bois RM

Subjects
Adult, Female, Hemolytic Plaque Technique, Humans, Male, Middle Aged, Up-Regulation, Leukocytes, Mononuclear metabolism, Macrophages, Alveolar metabolism, Sarcoidosis, Pulmonary metabolism, Tumor Necrosis Factor-alpha biosynthesis
Abstract

Tumour necrosis factor (TNF)-alpha is thought to be a key early cytokine in the pathogenesis of sarcoidosis, despite conflicting data. Largely the product of mononuclear phagocyte activation, it is unclear whether TNF-alpha production at disease sites is a feature of all mononuclear phagocytes that accumulate there or whether it is secreted by a subset of these cells. Using the reverse haemolytic plaque assay, the aims of this study were to determine if the upregulation of TNF-alpha could be confirmed and to investigate whether this was monocyte or macrophage specific. The reverse haemolytic plaque assay allows the measurement of cytokine production at a single cell level. A greater number of alveolar macrophages produced TNF-alpha compared to autologous monocytes in sarcoidosis but not in controls and, based on cell size, it was confirmed that this was the product of more mature macrophages and that the secretion of TNF-alpha by monocytes and macrophages was heterogeneous: not all monocytes and macrophages secrete TNF-alpha. No differences in the average levels of TNF-alpha secretion by peripheral blood monocytes or alveolar macrophages were observed. This study has demonstrated that a subset of mononuclear phagocytes, mature macrophages, are responsible for tumour necrosis factor secretion and this could have implications for targeted management in sarcoidosis in the future.

Published
2002
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