1. Effect of TongXie-YaoFang on Cl− and HCO3- Transport in Diarrhea-Predominant Irritable Bowel Syndrome Rats.
- Author
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Lu, Xiaofang, Zhang, Shengsheng, Yang, Cheng, Wang, Zhengfang, Zhao, Luqing, Wu, Zhenyu, and Xie, Jing
- Subjects
ANIMAL experimentation ,BIOLOGICAL models ,DIARRHEA ,HERBAL medicine ,INTESTINAL mucosa ,IRRITABLE colon ,CHINESE medicine ,RATS ,SEROTONIN - Abstract
TongXie-YaoFang (TXYF) can effectively alleviate the symptoms of diarrhea-predominant irritable bowel syndrome (D-IBS) patients. However, the curative mechanism has not been fully clarified. The study was designed to investigate the effect of TXYF on the colonic ion transport induced by serotonin (5-HT) in D-IBS rats. A method of multiple stress (neonatal maternal separation (NMS) combined with restraint stress (RS)) was used to induce the D-IBS model. The model rats were randomly divided into two groups: NMS + RS group and TXYF-formula group, and the normal control (no handling) rats were classified as NH group. In the NMS + RS group, the change of short-circuit current (ΔIsc) induced by 5-HT was lower than that in the NH and TXYF-formula groups. After removing of the extracellular Cl
− or HCO3 - or basolateral Na+ or blocking the cystic fibrosis transmembrane conductance regulator (CFTR), Na+ -K+ -2Cl− cotransporter (NKCC), Na+-HCO3 - cotransporter, Cl-/HCO3 - exchanger, K+ channel, or Na+ /K+ -ATPase, respectively, there was no difference in 5-HT-induced ΔIsc among the three groups. These data suggest that TXYF can regulate 5-HT-induced Cl− and HCO3 - secretion, possibly mediated by the combined action of CFTR, NKCC, Na+ -HCO3 - cotransporter, Cl-/HCO3 - exchanger, K+ channel, and Na+ /K+ -ATPase. [ABSTRACT FROM AUTHOR]- Published
- 2016
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