1. The path of carbon in photosynthesis. XX. The steady state
- Author
-
Peter Massini and Melvin Calvin
- Subjects
Pharmacology ,Inorganic chemistry ,chemistry.chemical_element ,Cell Biology ,Photosynthesis ,Carbon ,Carbon cycle ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,chemistry ,Carbon dioxide ,Respiration ,Molecular Medicine ,Carbon-14 ,Steady state (chemistry) ,Molecular Biology ,Phosphoglyceric acid - Abstract
The separation of the phenomenon of photosynthesis in green plants into a photochemical reaction and into the light-dependent reduction of carbon dioxide is discussed, The reduction of carbon dioxide and the fate of the assimilated carbon were investigated with the help of the tracer technique (exposure of the planks to the radioactive C{sup 14}O{sub 2}) and of paper chromatography. A reaction cycle is proposed in which phosphoglyceric acid is the first isolable assimilations product. Analyses of the algal extracts which had assimilated radioactive carbon dioxide in a stationary condition ('steady-state' photosynthesis) for a long time provided further information concerning the proposed cycle and permitted the approximate estimation, for a number of compounds of what fraction of each compound was taking part in the cycle. The earlier supposition that light influences the respiration cycle was confirmed. The possibility of the assistance of {alpha}-lipoic acid, or of a related substance, in this influence and in the photosynthesis cycle, is discussed.
- Published
- 1952