1. Effects of the antihypertensive drug benidipine on osteoblast function in vitro.
- Author
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BAIXIANG WANG, MING BI, ZHEN ZHU, LEI WU, and JINGYUN WANG
- Subjects
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HYPERTENSION , *THERAPEUTICS , *ANTIHYPERTENSIVE agents , *DIHYDROPYRIDINE , *CALCIUM antagonists , *OSTEOBLASTS , *ALKALINE phosphatase , *WESTERN immunoblotting - Abstract
The dihydropyridine-type calcium channel blocker, benidipine (BD) has been widely used in hypertension therapy. Previous studies have demonstrated that BD has a positive effect on bone metabolism. Inspired by this promoting phenomenon, the present study investigated the effects of BD on osteoblasts in vitro. Experiments were designed and performed, including an MTT assay, reverse transcription-polymerase chain reaction, western blot analysis, alkaline phosphatase activity measurements and alizarin red S staining. The results demonstrated that BD promoted osteoblast proliferation and osteogenic differentiation at concentrations from 1x10-6 to 1x10-9 M by upregulating Runx2, BMP2 and OCN gene expression levels. Overall, BD at appropriate concentrations has been demonstrated to have positive effects on osteoblast function in addition to its conventional clinical usage. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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