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Blood glutathione (GSH) and total glutathione (GSH plus GSSG) were determined in eleven patients during ACTH therapy and in eleven patients during the administration of cortisone. GSH values were significantly decreased from a pretreatment average of 36.6 mg per 100 cc to 26.9 mg by ACTH while cortisone produced a decrease from an average pretreatment value of 35.0 mg per 100 cc to 29.1 mg. The latter decrease, statistically, is of a low order of significance. The total glutathione values showed no changes following the administration of either ACTH or cortisone. During treatment with ACTH the greatest decrease in blood GSH occurred in the patients showing the greatest increase in blood sugar. There is suggestive evidence that although patients with normal glucose tolerance may show diabetic patterns during treatment, those who demonstrate mildly diabetic patterns prior to therapy show no further decrease in glucose tolerance and minimal reduction in GSH values after ACTH or cortisone therapy.

The proteins of whole egg, whole egg white, and yolk, were prepared and analyzed for cystine, methionine, phenylalanine, tryptophane, threonine, arginine, histidine, lysine, sulfur, and nitrogen. These proteins were incorporated into diets at a 10% level and fed to young rats. The protein efficiency ratio (gain in weight per gram of protein ingested) was determined after a 2-week feeding period. Commercial preparations of wmole egg and whole white protein were similarly analyzed and their PE ratio determined. The PE ratio of the whole white protein was higher than that of either the whole egg or the yolk proteins. The PE ratio of the commercial whole white protein was almost as high as that of the laboratory prepared sample. The PE ratio of commercial whole egg protein was markedly lower than that of the laboratory prepared sample. The amino acid content of all the whole egg proteins wTere quite similar with the exception of the cystine content of the commercial samples which was lower than that of the laboratory samples. When the commercial whole egg protein II was reenforced with 0.25% cystine the PE ratio was not improved. Other factors in addition to the amino acid content apparently play a role in the biological value of proteins.

Vesicular stomatitis virus, New Jersey type, was propagated in suspensions of surviving trypsin-dispersed kidney cells of guinea pigs as well as in cultivated epithelial cells and fibrocytes. Both production methods gave nearly equal virus yields, the highest being approximately 106789tissue culture ID50ml. The highest virus concentration in cultures and suspensions were most frequently recorded at 24 and 72 hours following infection, respectively. Cell suspensions were increased in volume from 2 ml to 200 ml without altering the per ml virus yields. After peak virus concentrations had been reached, the rate of destruction of virus infectivity upon further incubation was significantly higher for cultures than for suspensions. Vesicular stomatitis virus was also shown to grow in suspensions of dispersed bovine kidney cells.