1. Diverse hematopoietic potentials of five human embryonic stem cell lines
- Author
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Tatiana Ulyanova, Hua Cao, Jennifer Hesson, Thalia Papayannopoulou, Carol B. Ware, Angelique M. Nelson, Paul A. Fields, Kai Hsin Chang, and Betty Nakamoto
- Subjects
Cellular differentiation ,Biology ,Article ,Cell Line ,Mice ,Animals ,Humans ,Cell Lineage ,GATA1 Transcription Factor ,Globin ,Embryonic Stem Cells ,Genetics ,Gene Expression Profiling ,Cell Differentiation ,Karyotype ,Cell Biology ,Hematopoietic Stem Cells ,Phenotype ,Embryonic stem cell ,Coculture Techniques ,Hematopoiesis ,Cell biology ,Gene expression profiling ,Haematopoiesis ,Cell culture ,embryonic structures ,Biomarkers - Abstract
Despite a growing body of literature concerning the hematopoietic differentiation of human embryonic stem cells (hESCs), the full hematopoietic potential of the majority of existing hESC lines remains unknown. In this study, the hematopoietic response of five NIH-approved hESC lines (H1, hSF6, BG01, BG02, and BG03) was compared. Our data show that despite expressing similar hESC markers under self-renewing conditions and initiating mesodermal differentiation under spontaneous differentiation conditions, marked differences in subsequent hematopoietic differentiation potential among these lines existed. A high degree of hematopoietic differentiation was attained only by H1 and BG02, whereas this process appeared to be abortive in nature for hSF6, BG01, and BG03. This difference in hematopoietic differentiation predisposition was readily apparent during spontaneous differentiation, and further augmented under hematopoietic-inducing conditions. This predisposition appeared to be intrinsic to the specific hESC line and independent of passage number or gender karyotype. Interestingly, H1 and BG02 displayed remarkable similarities in their kinetics of hematopoietic marker expression, hematopoietic colony formation, erythroid differentiation, and globin expression, suggesting that a similar, predetermined differentiation sequence is followed. The identification of intrinsic and extrinsic factors governing the hematopoietic differentiation potential of hESCs will be of great importance for the putative clinical utility of hESC lines.
- Published
- 2008