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Choroidal neovascularization (CNV) is a hallmark of exudative age-related macular degeneration (exAMD) and a major cause of visual loss in AMD. Despite the widespread use of anti-VEGF therapy, serious adverse effects arise from repeated intravitreal injection of anti-VEGF antibodies, which warrant alternative strategy. We report herein that in a CNV murine model created by krypton red laser, intravenous injection of a serine racemase inhibitor, l-Aspartic acid β-hydroxamate (L-ABH), significantly reduced CNV at the dose 6 mg/kg on the first day before and followed by 3 mg/kg on the third day after laser injury. The CNV volumes were analyzed with isolectin GS-IB4 staining on choroidal/RPE flat mounts on the seventh day after laser injury. Injection of L-ABH did not produce negative effects on retinal function and visual behavior. To dissect the mechanism in vitro , pretreatment with L-ABH in primary RPE cultures significantly reduced production of vascular endothelial growth factor (VEGF) and macrophage chemotactic protein 1 (MCP-1) by TNFα-primed RPEs. Consistent with these observations, L-ABH pretreatment significantly attenuated macrophage migration mediated by TNFα-primed RPE. Collectively, intravenous injection of L-ABH significantly reduced CNV volumes via reducing production of VEGF and MCP-1 by inflammation-primed RPEs. We identify that intravenous injection of L-ABH significantly attenuates CNV by laser injury without negative effect on retinal function and visual behavior. The reducing effect on CNV is mediated via antagonizing VEGF and MCP-1 production by inflammation-primed RPEs. Image 1 • Intravenous injection of l-Aspartic acid β-hydroxamate (L-ABH) reduced choroidal neovascularization induced by laser injury. • Intravenous injection of L-ABH did not pose negative effect on retinal function and visual behavior. • Injection of L-ABH increased L-serine content and L/D-serine ratios in aqueous humor. • L-ABH inhibited production of MCP-1, VEGF by inflammation-primed RPEs. [ABSTRACT FROM AUTHOR]