1. IL-17A synergistically stimulates TNF-α-induced IL-8 production in human airway epithelial cells: A potential role in amplifying airway inflammation.
- Author
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Honda, Kojiro, Wada, Hiroo, Nakamura, Masuo, Nakamoto, Keitaro, Inui, Toshiya, Sada, Mitsuru, Koide, Takashi, Takata, Saori, Yokoyama, Takuma, Saraya, Takeshi, Kurai, Daisuke, Ishii, Haruyuki, Goto, Hajime, and Takizawa, Hajime
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EPITHELIAL cells ,ADRENOCORTICAL hormones ,NEUTROPHILS ,INFLAMMATION ,TUMOR necrosis factors ,MITOGEN-activated protein kinases ,INTERLEUKIN-17 ,INTERLEUKIN-8 - Abstract
Background:Recent reports have suggested an involvement of neutrophilic inflammation driven by interleukin (IL)-17 from Th17 cells, especially in severe, refractory asthma. It remains unknown about the possible interactions of this cytokine and other proinflammatory cytokines to direct neutrophilic airway inflammation.Materials and Methods:We evaluated the effects of IL-17A, IL-17E, and IL-17F in combination with other stimuli such as tumor necrosis factor (TNF) –α on the production and expression of IL-8 in human bronchial epithelial cells. We also studied their effects on other cytokine production. The possible role of mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways was evaluated by specific inhibitors. We examined the effects of anti-asthma drugs, such as steroids or salmeterol.Results:IL-17A alone induced only a minimal effect on IL-8 expression. IL-17A, but not IL-17E or IL-17F, in combination with TNF-α showed a synergistic effect on IL-8 expression. Similar findings were found when combination with IL-1β and IL-17A were used, but such was not the case with lipopolysaccharide (LPS). In addition, we further found such synergy on GM-CSF production. The synergy with TNF-α and IL-17A was significantly inhibited by MAPKs inhibitors. Corticosteroids such as fluticasone propionate and dexamethasone, but not salmeterol, partially suppressed the IL-17A and TNF-α-induced IL-8 production.Conclusions:IL-17A in the combination with TNF-α or IL-1β showed a synergistic augmenting effect on IL-8 and GM-CSF production in human airway epithelial cells. [ABSTRACT FROM PUBLISHER]
- Published
- 2016
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