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Start Over Author "Rassi, Yavar" Journal experimental parasitology
6 results on '"Rassi, Yavar"'

1. Anti Leishmania activity of Lucilia sericata and Calliphora vicina maggots in laboratory models

Author

Sanei-Dehkordi, Alireza, primary, Khamesipour, Ali, additional, Akbarzadeh, Kamran, additional, Akhavan, Amir Ahmad, additional, Mir Amin Mohammadi, Akram, additional, Mohammadi, Younes, additional, Rassi, Yavar, additional, Oshaghi, Mohammad Ali, additional, Alebrahim, Zahra, additional, Eskandari, Seyed Ebrahim, additional, and Rafinejad, Javad, additional

Published
2016
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3. Leishmania species: Detection and identification by nested PCR assay from skin samples of rodent reservoirs

Author

Akhavan, Amir Ahmad, primary, Mirhendi, Hossein, additional, Khamesipour, Ali, additional, Alimohammadian, Mohammad Hossein, additional, Rassi, Yavar, additional, Bates, Paul, additional, Kamhawi, Shaden, additional, Valenzuela, Jesus G., additional, Arandian, Mohammad Hossein, additional, Abdoli, Hamid, additional, Jalali-zand, Niloufar, additional, Jafari, Reza, additional, Shareghi, Niloufar, additional, Ghanei, Maryam, additional, and Yaghoobi-Ershadi, Mohammad Reza, additional

Published
2010
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4. Phlebotomus perfiliewi transcaucasicus is circulating both Leishmania donovani and L. infantum in northwest Iran

Author

Oshaghi, Mohammad Ali, primary, Ravasan, Naseh Maleki, additional, Hide, Mallorie, additional, Javadian, Ezat-Aldin, additional, Rassi, Yavar, additional, Sadraei, Javid, additional, Mohebali, Mehdi, additional, Mehdi Sedaghat, Mohammad, additional, Hajjaran, Homa, additional, Zarei, Zabiholah, additional, and Mohtarami, Fatemeh, additional

Published
2009
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6. Development of species-specific PCR and PCR-restriction fragment length polymorphism assays for L.infantum/L.donovani discrimination.

Author

Oshaghi MA, Ravasan NM, Hide M, Javadian EA, Rassi Y, Sedaghat MM, Mohebali M, and Hajjaran H

Subjects
Animals, Base Sequence, Cysteine Proteases genetics, Deoxyribonucleases, Type II Site-Specific, Dogs, Humans, Insect Vectors, Leishmania donovani enzymology, Leishmania donovani genetics, Leishmania infantum enzymology, Leishmania infantum genetics, Molecular Sequence Data, Psychodidae, Restriction Mapping, Species Specificity, Leishmania donovani isolation & purification, Leishmania infantum isolation & purification, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length
Abstract

Discrimination of Leishmania infantum and L. donovani, the members of the L. (L.) donovani complex, is important for diagnosis and epidemiological studies of visceral leishmaniasis (VL). We have developed two molecular tools including a restriction fragment length polymorphisms of amplified DNA (PCR-RFLP) and a PCR that are capable to discriminate L. donovani from L. infantum. Typing of the complex was performed by a simple PCR of cysteine protease B (cpb) gene followed by digestion with DraIII. The enzyme cuts the 741-bp amplicon of L. donovani into 400 and 341 bp fragments whereas the 702 bp of L. infantum remains intact. The designed PCR species-specific primer pair is specific for L. donovani and is capable of amplifying a 317 bp of 3' end of cpb gene of L. donovani whereas it does not generate an amplicon for L. infantum. The species-specific primers and the restriction enzyme were designed based on a 39 bp insertion/deletion (indel) in the middle of the cpb gene. Both assays could differentiate correctly the two species and are reliable and high-throughput alternatives for molecular diagnosis and epidemiological studies of VL in various foci.

Published
2009
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