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Your search keyword '"Sobhon, Prasert"' showing total 24 results
Start Over Author "Sobhon, Prasert" Journal experimental parasitology
24 results on '"Sobhon, Prasert"'

4. The in vitro anthelmintic effects of plumbagin on newly excysted and 4-weeks-old juvenile parasites of Fasciola gigantica

Author

Lorsuwannarat, Natcha, primary, Piedrafita, David, additional, Chantree, Pathanin, additional, Sansri, Veerawat, additional, Songkoomkrong, Sineenart, additional, Bantuchai, Sirasate, additional, Sangpairot, Kant, additional, Kueakhai, Pornanan, additional, Changklungmoa, Narin, additional, Chaichanasak, Pannigan, additional, Chansela, Piyachat, additional, and Sobhon, Prasert, additional

Published
2014
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5. Vaccine potential of recombinant cathepsin B against Fasciola gigantica

Author

Chantree, Pathanin, primary, Phatsara, Manussabhorn, additional, Meemon, Krai, additional, Chaichanasak, Pannigan, additional, Changklungmoa, Narin, additional, Kueakhai, Pornanan, additional, Lorsuwannarat, Natcha, additional, Sangpairoj, Kant, additional, Songkoomkrong, Sineenart, additional, Wanichanon, Chaitip, additional, Itagaki, Tadashi, additional, and Sobhon, Prasert, additional

Published
2013
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23. Fasciola gigantica: immunodiagnosis of fasciolosis by detection of circulating 28.5 kDa tegumental antigen.

Author

Anuracpreeda P, Wanichanon C, Chawengkirtikul R, Chaithirayanon K, and Sobhon P

Subjects
Animals, Antibodies, Helminth blood, Antigens, Helminth immunology, Antigens, Surface blood, Antigens, Surface immunology, Buffaloes, Cattle, Cricetinae, Cross Reactions, Cyprinidae, Enzyme-Linked Immunosorbent Assay methods, Lymnaea, Male, Mesocricetus, Mice, Mice, Inbred BALB C, Mice, Inbred ICR, Rabbits, Random Allocation, Sensitivity and Specificity, Antigens, Helminth blood, Fasciola immunology, Fascioliasis diagnosis
Abstract

A monoclonal antibody (MoAb)-based sandwich ELISA was developed for the detection of circulating 28.5 kDa tegumental antigen (28.5 kDa TA) in the sera from mice experimentally infected with Fasciola gigantica. The MoAb was immobilized on a microtiter plate, and the antigen in the serum was captured and detected with biotinylated polyclonal rabbit anti TA antibody. The test could detect 28.5 kDa in the extracts of tegument (TA), whole body (WB) and excretory-secretory (ES) fractions at the concentrations of these crude antigens as low as 600 pg/ml, 16 and 60 ng/ml, respectively. This sandwich ELISA assay could detect the infection from day 1 to 35 post infection and showed that circulating level of 28.5 kDa TA peaked at day 1 post infection. In contrast, the antibody detection by indirect ELISA could only demonstrate the antibody level from 35 days post infection. The reliability of the assay method was evaluated using sera from mice infected with F. gigantica or Schistosoma mansoni, and hamsters infected with Opisthorchis viverrini, as well as healthy mice and hamsters. The sandwich ELISA exhibited a sensitivity and specificity at 94.55% and 100%, respectively, and with a positive predictive value of 100%, a negative predictive value of 97.39%, false positive rate of 0%, false negative rate of 5.50% and an accuracy of 98.2%. Thus, this detection method exhibited high specificity and sensitivity as well as could be used for early diagnosis of fasciolosis by F. gigantica.

Published
2009
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24. Fasciola gigantica: immunolocalization of 28.5 kDa antigen in the tegument of metacercaria and juvenile fluke.

Author

Anuracpreeda P, Wanichanon C, and Sobhon P

Subjects
Animals, Antibodies, Monoclonal biosynthesis, Antigens, Surface analysis, Cricetinae, Female, Fluorescent Antibody Technique, Indirect, Immunoenzyme Techniques, Immunohistochemistry, Lymnaea, Male, Mesocricetus, Mice, Mice, Inbred BALB C, Microscopy, Electron, Transmission, Time Factors, Antigens, Helminth analysis, Fasciola growth & development, Fasciola immunology, Life Cycle Stages immunology
Abstract

Specific monoclonal antibody (MoAb) to 28.5 kDa tegumental antigen (TA) was used to localize this antigen in the tissues of metacercariae, newly excysted juvenile (NEJ), 1, 3, 5, and 7-week-old juveniles of Fasciola gigantica by using indirect immunofluorescence, immunoperoxidase and immunogold techniques. Both indirect immunofluorescence and immunoperoxidase detections showed that this antigen was concentrated in the tegument particularly in its outer rim, tegumental cells and their processes as well as epithelial linings of the oral sucker. Unlike adult F. gigantica, it was not detected in spermatogenic cells in the testes, cells of Mehlis'gland, oocytes within the ovary, and ovum within the egg of parasites. At the ultrastructural level, the immunogold labeling showed deposit of gold particles specifically in G2 tegumental granules and on the surface membrane. Thus, this 28.5 kDa antigen is expressed in the tegument and associated structures of juvenile parasites, and it could be a major component of the G2 granules which are shown to fuse with the surface membrane and contribute material to replace the casted-off membrane. This process is the replenishment and turnover of the surface membrane to prevent the attachment of the host immune effector cells.

Published
2009
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