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Background: The COVID-19 pandemic is increasing rapidly. Hospital acquired infections enhance local outbreaks, impacting the vulnerable populations. Infection prevention and control practices (IPC) refer to all the activities used to reduce the risk of infection spread. This study aims to estimate the proportion of health care workers (HCWs) who acquired SARS-CoV2 infection, and evaluate their knowledge to IPC and suggest recommendations to reduce the risk of SARS-CoV2 infection. Methods: This is a cross-sectional study conducted in Egypt from June 8, 2020, till August 19, 2020. A purposive sample of 518 HCWs from different governorates was included in the study. HCWs filled a structured questionnaire developed by the World Health Organization on a Google Form and a printed copy. The questionnaire link was shared on social media forums including HCWs such as Facebook. Results: The mean age of HCWs was 33±7 and 65% were males. The majority of health care workers were affiliated with the Ministry of Health and Population (62.5%), recruited from Cairo (52.4%), and were physicians (78.2%). About 11% of the HCWs had been infected with SARS-CoV2. Their mean knowledge percent score regarding IPC was 36.19 ± 11.26. The highest rate of infection was among those with little experience (p=0.002), and those worked inside Cairo (p=0.018). About 89% mentioned that the leading cause of infection with SARS-CoV2 was the shortage in Personal Protective Equipment (PPE). About 42% recommended raising the awareness for the importance of IPC to decrease risk of infection. Conclusions: HCWs had a low score of knowledge toward IPC. There was a considerable proportion of SARS-CoV2 infection among them. Lack of knowledge and shortage of PPE were the contributing factors. It is necessary to provide juniors with IPC training, and hospitals with sufficient PPE.