1. Autocrine Action of NAD(P)H Oxidase-Derived Extracellular Superoxide in Coronary Arterial Myocytes.
- Author
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Fan Zhang, Qi-Zheng Chen, Fan Yi, and Pin-Lan Li
- Subjects
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AUTOCRINE mechanisms , *PARACRINE mechanisms , *SUPEROXIDES , *NAD(P)H dehydrogenases , *OXIDASES , *MUSCLE cells , *SUPEROXIDE dismutase , *RYANODINE - Abstract
Our recent studies have indicated that there is an autocrine/paracrine pattern of superoxide production through NAD(P)H oxidase in coronary arterial myocytes (CAMs). The present study determined whether extracellular O2*- could serve as an autocrine factor to activate intracellular Ca2+ signaling pathway and explored related underlying mechanisms. Using a newly established dual fluorescence microscopic imaging technique, We found that M1-receptor agonist oxotremorine (Oxo) simultaneously increased the extracellular O2*- production and [Ca2+]i,, which were significantly attenuated by addition of superoxide dismutase (SOD) and catalase into the bath solution. NAD(P)H oxidase inhibitor, apocynin also inhibited this response of CAMs to Oxo. When ADP-ribosyl cyclase inhibitor -- nicotinamide, RyR/ Ca2+release channel antagonists -- ryanodine or cADPR antagonist -- 8-Br-cADPR was added into the bath solution, Oxo-induced increase in [Ca2+]i was abolished, while extracellular O2*- production remained. Furthermore, introduction of cADPR into CAMs by ultrasound microbubbles increased [Ca2+]i,,but it had no effect on extracellular O2*- production. These results provide direct evidence that extracellular O2*- derived from NAD(P)H oxidase contributes to Ca2+activation of CAMs through its autocrine action on cADPR signaling pathway (Supported by NIH grants HL057244 and HL075316). [ABSTRACT FROM AUTHOR]
- Published
- 2007
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