1. The PDZ/coiled-coil domain containing protein PIST modulates insulin secretion in MIN6 insulinoma cells by interacting with somatostatin receptor subtype 5
- Author
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Heike Zitzer, Jesper Gromada, Hans-Jürgen Kreienkamp, Wolf Wente, Iris Treinies, Alexander M. Efanov, and Dietmar Richter
- Subjects
endocrine system ,medicine.medical_specialty ,media_common.quotation_subject ,PDZ domain ,Biophysics ,Somatostatin subtype 5 receptor ,Biology ,Biochemistry ,Green fluorescent protein ,Mice ,PIST ,Structural Biology ,Internal medicine ,Insulin-Secreting Cells ,Insulin Secretion ,Genetics ,medicine ,Animals ,Humans ,Insulin ,Receptors, Somatostatin ,Rats, Wistar ,education ,Internalization ,Molecular Biology ,Insulinoma ,media_common ,G protein-coupled receptor ,education.field_of_study ,Somatostatin receptor-5 ,Somatostatin receptor ,Cell Membrane ,Membrane Proteins ,Cell Biology ,medicine.disease ,Cell biology ,Protein Structure, Tertiary ,Rats ,Endocrinology ,Somatostatin ,Glucose ,Islets ,Carrier Proteins ,hormones, hormone substitutes, and hormone antagonists - Abstract
The multi-domain protein PIST (protein interacting specifically with Tc10) interacts with the SSTR5 (somatostatin receptor 5) and is responsible for its intracellular localization. Here, we show that PIST is expressed in pancreatic β-cells and interacts with SSTR5 in these cells. PIST expression in MIN6 insulinoma cells is reduced by somatostatin (SST). After stimulation with SST, SSTR5 undergoes internalization together with PIST. MIN6 cells over-expressing PIST display enhanced glucose-stimulated insulin secretion and a decreased sensitivity to SST-induced inhibition of insulin secretion. These data suggest that PIST plays an important role in insulin secretion by regulating SSTR5 availability at the plasma membrane.
- Published
- 2005