1. Specific detections of the early process of the glycation reaction by fructose and glucose in diabetic rat lens
- Author
-
Ayumu Hoshi, Yasuo Tano, Nobuko Miyazawa, Naoyuki Taniguchi, Junichi Fujii, Yoshimi Kawasaki, and Ayako Okado
- Subjects
Crystallin ,Glycosylation ,Time Factors ,Biophysics ,Fructose ,Carbohydrate metabolism ,Biochemistry ,Diabetes Mellitus, Experimental ,chemistry.chemical_compound ,Polyol pathway ,Structural Biology ,Glycation ,Reference Values ,Amadori rearrangement ,Diabetes mellitus ,Lens, Crystalline ,Genetics ,medicine ,Animals ,Platelet Activating Factor ,Molecular Biology ,Lysine ,Cell Biology ,medicine.disease ,Crystallins ,Rats ,Glucose ,chemistry ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Sorbitol ,Glucation ,Amadori product ,Fructation - Abstract
The glycation reaction by fructose, as well as that by glucose, in control and diabetic rat lens was analyzed by using antibodies which specifically recognize adducts of lysine with fructose and with glucose. Levels of fructose adducts in diabetic rat lens were 2.5 times that of the control, and correlated with sorbitol levels. This was mainly due to enhanced glycation of β- and γ-crystallins by fructose under diabetic conditions. These data suggest that glycation by fructose may also play a role in cataract formation under conditions of diabetes and aging.
- Published
- 1999