1. The heterogeneous nuclear ribonucleoprotein K is important for Herpes simplex virus-1 propagation.
- Author
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Schmidt T, Striebinger H, Haas J, and Bailer SM
- Subjects
- Animals, Blotting, Western, Chlorocebus aethiops, DNA, Viral genetics, HeLa Cells, Herpesvirus 1, Human genetics, Herpesvirus 1, Human metabolism, Heterogeneous-Nuclear Ribonucleoprotein K genetics, Humans, Microscopy, Fluorescence, Polymerase Chain Reaction, Vero Cells, Viral Proteins genetics, Viral Proteins metabolism, Virus Replication genetics, Herpesvirus 1, Human growth & development, Heterogeneous-Nuclear Ribonucleoprotein K metabolism, RNA Interference physiology, Virus Replication physiology
- Abstract
The heterogeneous nuclear ribonucleoprotein (hnRNP) K is an evolutionarily conserved protein with roles in signal transduction and gene expression. An impact of hnRNP K on the life cycle of a broad range of viral pathogens was reported while functional data for herpesviruses were lacking. In this study we show that hnRNP K is important for Herpes simplex virus 1 egress. In absence of hnRNP K, viral entry, gene expression, viral DNA replication, and maturation of nuclear particles appear normal whereas release of infectious virions to the extracellular space was significantly affected. Our results indicate that hnRNP K has an impact on a late step of herpesviral propagation making it a potential antiviral target., (Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.) more...
- Published
- 2010
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