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Your search keyword '"Dikanov, Sergei A."' showing total 5 results
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5 results on '"Dikanov, Sergei A."'

4. Two-dimensional pulsed electron spin resonance characterization of 15N-labeled archaeal Rieske-type ferredoxin

Author

Iwasaki, Toshio, Samoilova, Rimma I., Kounosu, Asako, and Dikanov, Sergei A.

Subjects
ELECTRON nuclear double resonance, ELECTRON spin echo envelope modulation spectroscopy, ELECTRON paramagnetic resonance, NUCLEAR magnetic resonance, FOURIER transform optics
Abstract

Abstract: Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly 15N-labeled archaeal Rieske-type [2Fe–2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high-potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non-coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems. [Copyright &y& Elsevier]

Published
2009
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5. X‐band ESEEM spectroscopy of 15N substituted native and inhibitor‐bound superoxide dismutase

Author

Dikanov, Sergei, Felli, Isabella, Viezzoli, Maria-Silvia, Spoyalov, Andrei, and Hüttermann, Jürgen

Abstract

The hyperfine couplings of the remote nitrogens of histidine ligands are determined for the first time by an X‐band ESEEM spectroscopy study of 15N‐substituted superoxide dismutase (SOD). They show a significant difference between two groups of ligands with different orientation relative to the metal ion. The ESEEM spectra of 15N SOD with cyanide as an inhibitor containing 14N and 15N are also discussed. They allow some conclusions to be drawn about structural changes upon inhibitor binding and indicate the necessity of further multifrequency investigations.

Published
1994
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