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Your search keyword '"Filip V. Toukach"' showing total 3 results
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3 results on '"Filip V. Toukach"'

1. Structural and serological studies of the O-specific polysaccharide of the bacteriumProteus mirabilisO10 containing<scp>L</scp>-altruronic acid, a new component of O-antigens

Author

Nikolay A. Paramonov, Sof'ya N. Senchenkova, Filip V. Toukach, Anna St. Swierzko, Andrzej Ziółkowski, Alexander S. Shashkov, Yuriy A. Knirel, Maciej Cedzynski, and W. Kaca

Subjects
Glycosylation, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Biophysics, Disaccharide, Enzyme-Linked Immunosorbent Assay, Bacterial polysaccharide structure, Cross Reactions, Hemolysis, Biochemistry, Epitopes, chemistry.chemical_compound, Residue (chemistry), Lipopolysaccharide serological specificity, Structural Biology, Genetics, Humans, Tetrasaccharide, Proteus mirabilis, Molecular Biology, l-Altruronic acid, biology, O Antigens, Cell Biology, Nuclear magnetic resonance spectroscopy, biology.organism_classification, Antibodies, Bacterial, Precipitin Tests, Proteus, Uronic Acids, Carbohydrate Sequence, chemistry, d-Galacturonic acid, Oxidation-Reduction, D-Galacturonic acid, Bacteria
Abstract

An acidic O-specific polysaccharide from the lipopolysaccharide of Proteus mirabilis O10 contains 2-acetamido-2deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, d-galacturonic acid, and l-altruronic acid, the last-named sugar having not been found hitherto in O-antigens. Structure of a branched tetrasaccharide repeating unit of the polysaccharide was established by 1H and 13C NMR spectroscopy, including two-dimensional COSY and rotating-frame NOE spectroscopy. The lateral l-altruronic acid residue plays the immunodominant role in manifestation of the O10 specificity of Proteus, whereas a disaccharide fragment of the main chain in common with the O-specific polysaccharide of P. mirabilis O43 provides the one-way serological cross-reactivity between anti-O10 serum and O43-antigen.

Published
1996
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2. Structures of new acidic O-specific polysaccharides of the bacterium Proteus mirabilis serogroups O26 and O30

Author

Nikolay A. Paramonov, Yuriy A. Knirel, Alexander S. Shashkov, Andrzej Ziółkowski, Filip V. Toukach, Sof'ya N. Senchenkova, and W. Kaca

Subjects
Magnetic Resonance Spectroscopy, Lipopolysaccharide, Stereochemistry, Molecular Sequence Data, Biophysics, Polysaccharide, Biochemistry, chemistry.chemical_compound, O-Antigen structure, Structural Biology, Amide, Carbohydrate Conformation, Genetics, Tetrasaccharide, Serotyping, Sugar, Molecular Biology, Proteus mirabilis, chemistry.chemical_classification, biology, Bacterial acidic polysaccharide, O Antigens, Cell Biology, Hexuronic acid, biology.organism_classification, bacterial infections and mycoses, Carbohydrate Sequence, chemistry, l-Lysine, bacteria, Two-dimensional nuclear magnetic resonance spectroscopy, Bacteria
Abstract

The polysaccharide chains of the lipopolysaccharides of the Proteus mirabilis serogroups O26 and O30 were studied using sugar and methylation analysis and 1H and 13C NMR spectroscopy, including two-dimensional correlation spectroscopy and rotating-frame NOE spectroscopy. The polysaccharides were found to be acidic due to the presence of d-galacturonic acid and its amide with l-lysine in serogroup O26 or d-glucuronic acid in serogroup O30, and the structures of their tetrasaccharide repeating units were established. The O26-specific polysaccharide is structurally and serologically related to the O-specific polysaccharide of P. mirabilis O28, which includes amides of d-GalA with l-lysine and l-serine [Radziejewska-Lebrecht, J. et al. (1995) Eur. J. Biochem. 230, 705–712].

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3. Structures of the O-antigens of Proteus bacilli belonging to OX group (serogroups O1–O3) used in Weil-Felix test

Author

Anna St. Swierzko, Filip V. Toukach, Maciej Cedzynski, W. Kaca, Andrzej Ziółkowski, Alexander S. Shashkov, Sof'ya N. Senchenkova, Ken-ichi Amano, and Yuriy A. Knirel

Subjects
Bacilli, Magnetic Resonance Spectroscopy, Proteus vulgaris, Molecular Sequence Data, Biophysics, Oligosaccharides, Lipopolysaccharide, Cross Reactions, Bacterial polysaccharide structure, Rickettsiosis, Weil-Felix test, Biochemistry, Microbiology, Antigen, Structural Biology, Group (periodic table), Genetics, medicine, Carbohydrate Conformation, Humans, Serologic Tests, Rickettsia, Molecular Biology, Proteus mirabilis, biology, Monosaccharides, O Antigens, Rickettsia Infections, Cell Biology, O-antigen, biology.organism_classification, medicine.disease, Proteus, Antibodies, Bacterial, Weil–Felix test, O-Antigens, Carbohydrate Sequence, Serological cross-reactivity, Sugar Phosphates
Abstract

Structures of the O-specific polysaccharide chains of lipopolysaccharides from Proteus group OX strains (serogroups O1–O3) used as antigens in Weil-Felix test for diagnosis of rickettsiosis, were established. From them, the acid-labile polysaccharide of Proteus vulgaris OX19 (O1) is built up of the following branched pentasaccharide repeating units connected via a phosphate group: Download : Download full-size image where QuiNAc stands for 2-acetamido-2,6-dideoxyglucose (N-acetylquinovosamine). The basis of serospecificity of the Proteus group OX antigens and their cross-reactivity with human anti-rickettsial antibodies is discussed.

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