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1. Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis.

Author

Mourad GS, Tippmann-Crosby J, Hunt KA, Gicheru Y, Bade K, Mansfield TA, and Schultes NP

Subjects
Amino Acid Sequence, Arabidopsis drug effects, Arabidopsis growth & development, Arabidopsis Proteins chemistry, Biological Transport, Genetic Loci genetics, Molecular Sequence Data, Mutagenesis, Insertional, Nucleobase Transport Proteins, Purines chemistry, Purines toxicity, Pyrimidines chemistry, Pyrimidines toxicity, Sequence Alignment, Symporters chemistry, Arabidopsis genetics, Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism, Symporters genetics, Symporters metabolism
Abstract

Locus At5g03555 encodes a nucleobase cation symporter 1 (AtNCS1) in the Arabidopsis genome. Arabidopsis insertion mutants, AtNcs1-1 and AtNcs1-3, were used for in planta toxic nucleobase analog growth studies and radio-labeled nucleobase uptake assays to characterize solute transport specificities. These results correlate with similar growth and uptake studies of AtNCS1 expressed in Saccharomyces cerevisiae. Both in planta and heterologous expression studies in yeast revealed a unique solute transport profile for AtNCS1 in moving adenine, guanine and uracil. This is in stark contrast to the canonical transport profiles determined for the well-characterized S. cerevisiae NCS1 proteins FUR4 (uracil transport) or FCY2 (adenine, guanine, and cytosine transport)., (Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)

Published
2012
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2. AtAzg1 and AtAzg2 comprise a novel family of purine transporters in Arabidopsis.

Author

Mansfield TA, Schultes NP, and Mourad GS

Subjects
Adenine analogs & derivatives, Amino Acid Sequence, Arabidopsis genetics, Azaguanine metabolism, Biological Transport, Molecular Sequence Data, Nucleobase Transport Proteins classification, Nucleobase Transport Proteins genetics, Phylogeny, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Adenine metabolism, Arabidopsis metabolism, Guanine metabolism, Nucleobase Transport Proteins physiology
Abstract

In plants, nucleobase biochemistry is highly compartmented relying upon a well-regulated and selective membrane transport system. In Arabidopsis two proteins, AtAzg1 and AtAzg2, show substantial amino acid sequence similarity to the adenine-guanine-hypoxanthine transporter AzgA of Aspergillus nidulans. Analysis of single and double mutant lines harboring T-DNA insertion alleles AtAzg1-1 and AtAzg2-1 reveal a marked resistance to growth in the presence of 8-azaadenine and 8-azaguanine but not to other toxic nucleobase analogues. Conversely, yeast strains expressing AtAzg1 and AtAzg2 gain heightened sensitivity to growth on 8-azaadenine and 8-azaguanine. Radio-labeled purine uptake experiments in yeast and in planta confirm the function of AtAzg1 and AtAzg2 as plant adenine-guanine transporters.

Published
2009
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