1. The PsbS protein controls the macro-organisation of photosystem II complexes in the grana membranes of higher plant chloroplasts
- Author
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Egbert J. Boekema, Roman Kouril, Sami Kereiche, Peter Horton, Anett Z. Kiss, Groningen Biomolecular Sciences and Biotechnology, and Electron Microscopy
- Subjects
Chloroplasts ,Light ,Photosystem II ,PH-DEPENDENT DISSIPATION ,GREEN PLANTS ,Arabidopsis ,Light-Harvesting Protein Complexes ,Biophysics ,RHODOBACTER-SPHAEROIDES ,Biology ,EXCITATION-ENERGY ,Photosynthesis ,PHOTOPROTECTIVE ENERGY-DISSIPATION ,Thylakoids ,Biochemistry ,Thylakoid membrane ,Light-harvesting complex ,Photosystem II supercomplex ,Rhodobacter sphaeroides ,Structural Biology ,Genetics ,THYLAKOID MEMBRANES ,ANTENNA ,Molecular Biology ,Arabidopsis Proteins ,Non-photochemical quenching ,PsbS ,LIGHT-HARVESTING COMPLEXES ,Photosystem II Protein Complex ,food and beverages ,Cell Biology ,IN-VITRO ,biology.organism_classification ,Chloroplast ,Microscopy, Electron ,Membrane ,Thylakoid ,Mutation ,Crystallization ,PHOTOSYNTHETIC MEMBRANES - Abstract
The PsbS protein is a critical component in the regulation of non-photochemical quenching (NPQ) in higher plant photosynthesis. Electron microscopy and image analysis of grana membrane fragments from wild type and mutant Arabidopsis plants showed that the semi-crystalline domains of photosystem II supercomplexes were identical in the presence and absence of PsbS. However, the frequency of the domains containing crystalline arrays was increased in the absence of PsbS. Conversely, there was a complete absence of such arrays in the membranes of plants containing elevated amounts of this protein. It is proposed that PsbS controls the macro-organisation of the grana membrane, providing an explanation of its role in NPQ. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
- Published
- 2010