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2 results on '"Phospholipase Cδ1"'

1. Phospholipase Cδ1 does not mediate Ca2+ responses in neonatal rat cardiomyocytes

Author

Woodcock, Elizabeth A., Mitchell, Christopher J., and Biden, Trevor J.

Subjects
*PHOSPHOLIPASE C, *ADENOVIRUSES
Abstract

Phospholipase C (PLC) activation in neonatal rat ventricular cardiomyocytes (NRVM) generates inositol(1,4,5)trisphosphate (Ins(1,4,5)P3) in response to elevations in Ca2+ or inositol(1,4)bisphosphate in response to G protein stimulation. Overexpression of PLCδ1 increased total [3H]inositol phosphate (InsP) content and elevated [3H]Ins(1,4,5)P3, but failed to increase [3H]InsP responses to the Ca2+ ionophore A23187. Antisense PLCδ1 expression reduced endogenous PLCδ1 content but did not decrease the A23187 response. In permeabilized NRVM, [3H]InsP responses to elevated Ca2+ were not inhibited by Ins(1,4,5)P3, even at concentrations 1000-fold greater than required for selective inhibition of PLCδ1. Taken together these data provide evidence that PLCδ1 does not mediate the InsP response to elevated Ca2+ in NRVM. [Copyright &y& Elsevier]

Published
2003
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2. Phospholipase Cdelta(1) does not mediate Ca(2+) responses in neonatal rat cardiomyocytes

Author

Elizabeth A. Woodcock, Chris J. Mitchell, and Trevor J. Biden

Subjects
medicine.medical_specialty, G protein, Biophysics, Stimulation, Endogeny, Cardiomyocyte, Inositol 1,4,5-Trisphosphate, Phospholipase, Biology, Biochemistry, Rats, Sprague-Dawley, chemistry.chemical_compound, Phospholipase C delta, Structural Biology, Internal medicine, Genetics, medicine, Adenovirus, Animals, Inositol, Inositol phosphate, Molecular Biology, Cells, Cultured, chemistry.chemical_classification, Phospholipase C, Myocardium, Cell Biology, Phospholipase Cδ1, Rats, Isoenzymes, Endocrinology, chemistry, Animals, Newborn, Type C Phospholipases, Calcium
Abstract

Phospholipase C (PLC) activation in neonatal rat ventricular cardiomyocytes (NRVM) generates inositol(1,4,5)trisphosphate (Ins(1,4,5)P(3)) in response to elevations in Ca(2+) or inositol(1,4)bisphosphate in response to G protein stimulation. Overexpression of PLCdelta(1) increased total [(3)H]inositol phosphate (InsP) content and elevated [(3)H]Ins(1,4,5)P(3), but failed to increase [(3)H]InsP responses to the Ca(2+) ionophore A23187. Antisense PLCdelta(1) expression reduced endogenous PLCdelta(1) content but did not decrease the A23187 response. In permeabilized NRVM, [(3)H]InsP responses to elevated Ca(2+) were not inhibited by Ins(1,4,5)P(3), even at concentrations 1000-fold greater than required for selective inhibition of PLCdelta(1). Taken together these data provide evidence that PLCdelta(1) does not mediate the InsP response to elevated Ca(2+) in NRVM.

Published
2003

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