1. The oocyte-specific transcription factor, Nobox, regulates the expression of Pad6, a peptidylarginine deiminase in the oocyte
- Author
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Mikyung Choi, Aleksandar Rajkovic, Youngsok Choi, Dong Ryul Lee, Miseon Park, Ok-Hee Lee, Jeong-Jae Ko, Sanghyun Jeon, and Tae Ki Yoon
- Subjects
Transcriptional Activation ,Hydrolases ,Recombinant Fusion Proteins ,Biophysics ,Regulator ,Biology ,In Vitro Techniques ,Nobox ,Biochemistry ,Mice ,Protein-Arginine Deiminase Type 6 ,Oogenesis ,Structural Biology ,Transcription (biology) ,Genetics ,Transcriptional regulation ,medicine ,Animals ,Pad6 ,Promoter Regions, Genetic ,Molecular Biology ,Gene ,Transcription factor ,DNA Primers ,Homeodomain Proteins ,Mice, Knockout ,Base Sequence ,Ovary ,Gene Expression Regulation, Developmental ,Cell Biology ,Oocyte ,Molecular biology ,Embryonic stem cell ,Folliculogenesis ,medicine.anatomical_structure ,Animals, Newborn ,Oocytes ,Protein-Arginine Deiminases ,Female ,Transcription ,Transcription Factors - Abstract
Nobox is an oocyte-specific transcriptional regulator. Nobox deficiency disrupts early folliculogenesis and the expression of oocyte-specific genes in mice. In the present study, we found that peptidylarginine deiminase 6 (Pad6) was downregulated in Nobox-null ovaries. Pad6 is preferentially expressed in oocytes and its transcript is detectable at embryonic day 16.5. In addition, we identified one Nobox DNA-binding element (NBE) within the mouse Pad6 promoter. The NBE includes a core sequence TAATTA. Sequence-specific binding of Nobox to the TAATTA motif was confirmed. Nobox overexpression augmented transcriptional activity of a luciferase reporter driven by mouse Pad6. Our findings indicate that Nobox is a critical regulator that orchestrates oocyte-specific genes such as Pad6 during folliculogenesis.
- Published
- 2010