1. Drosophila ferritin mRNA: alternative RNA splicing regulates the presence of the iron-responsive element
- Author
-
Öjar Melefors, Sophia K. Ekengren, Kenneth Söderhäll, and Maria I. Lind
- Subjects
Iron-Sulfur Proteins ,DNA, Complementary ,Transcription, Genetic ,Iron ,Molecular Sequence Data ,Biophysics ,Exonic splicing enhancer ,RNA-binding protein ,Biology ,Primary transcript ,Biochemistry ,Exon ,Structural Biology ,Sequence Homology, Nucleic Acid ,Receptors, Transferrin ,Genetics ,Animals ,Humans ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Molecular Biology ,Genomic Library ,Ferritin ,Alternative RNA splicing ,Alternative splicing ,Intron ,Gene Expression Regulation, Developmental ,Iron-Regulatory Proteins ,RNA-Binding Proteins ,Cell Biology ,Molecular biology ,Recombinant Proteins ,Cell biology ,Post-transcriptional modification ,Alternative Splicing ,Drosophila melanogaster ,RNA splicing ,Ferritins ,Iron-responsive element ,Nucleic Acid Conformation ,Sequence Alignment - Abstract
Several mRNAs encoding the same ferritin subunit of Drosophila melanogaster were identified. Alternative RNA splicing and utilisation of different polyadenylation sites were found to generate the transcripts. The alternative RNA splicing results in ferritin transcripts with four unique 5′ untranslated regions. Only one of them contains an iron-responsive element. The iron-responsive element was found to bind in vitro specifically to human recombinant iron regulatory protein 1. Furthermore, the ferritin subunit mRNAs are differentially expressed during development. Our data provides the first molecular evidence that the presence of iron-responsive element in a ferritin mRNA is regulated by alternative RNA splicing.
- Full Text
- View/download PDF