Search

Your search keyword '"Wieloch, T"' showing total 5 results
Start Over Author "Wieloch, T" Journal febs letters
5 results on '"Wieloch, T"'

3. Trypsin activation of porcine procolipase. Kinetics of activation and effects on lipid binding.

Author

Wieloch T

Subjects
Animals, Enzyme Precursors, Hydrogen-Ion Concentration, Kinetics, Micelles, Swine, Taurodeoxycholic Acid metabolism, Colipases metabolism, Protein Precursors metabolism, Proteins metabolism, Triglycerides metabolism, Trypsin pharmacology
Abstract

The kinetics of trypsin activation of pancreatic procolipase was investigated and the pH dependence of the binding of procolipase and colipase to a tributyrine-bile salt interface studied. The Km was 0.06 mM and kcat 8 s-1, and was of the same order of magnitude as for the activation of pancreatic zymogens. At basic pH values colipase had a higher affinity for the tributyrine-bile salt interface as compared to procolipase. The trypsin activation of procolipase ensures a rapid degradation of dietary lipids in the intestine.

Published
1985
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results