1. Elevated citrate levels in non-alcoholic fatty liver disease: the potential of citrate to promote radical production.
- Author
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van de Wier B, Balk JM, Haenen GR, Giamouridis D, Bakker JA, Bast BC, den Hartog GJ, Koek GH, and Bast A
- Subjects
- Aged, Female, Hep G2 Cells, Humans, Hydrogen Peroxide metabolism, Male, Middle Aged, Non-alcoholic Fatty Liver Disease, Oxidative Stress, Citric Acid blood, Fatty Liver blood
- Abstract
Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated hydrogen peroxide induced intracellular oxidative stress in HepG2 cells. This was related to the promotion of iron mediated hydroxyl radical formation from hydrogen peroxide by citrate. The stimulating effect of citrate on the reactivity of iron promotes oxidative stress, a crucial process in the progression of NAFLD., (Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Published
- 2013
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