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Alginate oligosaccharides are a kind of low molecular weight polymer composed of 2~10 monosaccharide molecules linked by the same or different glycosidic bonds. Alginate lyase is an important tool for the enzymatic preparation of AOS. AOS prepared by the enzymatic method is superior to AOS prepared by other methods in purity, performance and structure. In this paper, the origin, structure and enzymatic properties of alginate lyase were reviewed, and the applications of alginate lyase in aquaculture, such as feed additive, microalgae cultivation, immune regulation, antioxidant and antibacterial, were summarized. The research direction of alginate lyase and the application prospect of alginate lyase in aquaculture were prospected, which provided reference for the application of alginate lyase and AOS in aquaculture. [ABSTRACT FROM AUTHOR]

The experiment was conducted to study the degradation rate of RPCys and RPMet in saliva, rumen and small intestine, examine the stability of RPCys and RPMet coating. The degradation rates of RPCys and RPMet were determined by artificial saliva method, semi-internal nylon bag method and in vitro simulated intestinal digestion method, and the protective effect of RPCys and RPMet over rumen was evaluated comprehensively. The results showed that the degradation rates of RPCys and RPMet in artificial saliva were 3.24%, 9.32% in 2 h and 4.10%, 11.84% in 4 h, respectively. The effective degradation rates in rumen were 8.97% and 32.00%, respectively. The degradation rate was higher than 87.38% and 81.12% after 2 h in the simulated small intestine environment in vitro, respectively. The experiment indicates that RPCys and RPMet are well coated, stable in artificial saliva and rumen, which have high small intestinal release rate. Compared with RPMet, RPCys has better rumen bypass protection effect. [ABSTRACT FROM AUTHOR]