1. A new cordycepin-producing caterpillar fungus Ophiocordyceps xuefengensis with artificial infection to the host, cultivation of mycelia and stromata
- Author
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Lin Chen, Shuihan Zhang, Dan Wan, Jian Jin, You Qin, Bingbing Shen, Cai Yuan, Lanping Zhen, Yuhui Qin, and Can Zhong
- Subjects
0106 biological sciences ,0301 basic medicine ,Adenosine ,Biology ,DNA, Ribosomal ,01 natural sciences ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,010608 biotechnology ,Botany ,Genetics ,medicine ,Animals ,Caterpillar ,Molecular Biology ,Mycelium ,Deoxyadenosines ,Cordycepin ,Strain (chemistry) ,Host (biology) ,fungi ,biology.organism_classification ,Culture Media ,Lepidoptera ,030104 developmental biology ,chemistry ,Ophiocordyceps xuefengensis ,Larva ,Fermentation ,Hypocreales ,medicine.drug - Abstract
Caterpillar fungi have numerous pharmacological and therapeutic applications in traditional medicine, due to a variety of active chemical constituents, such as cordycepin and adenosine. It is imperative to discover new resource for artificial cultivation and biometabolite production since the traditional natural species are endangered. In this study, a new strain HACM 001 was isolated and identified as Ophiocordyceps xuefengensis by rDNA-ITS sequencing. This strain showed the potential of artificial infection to caterpillar larvae leading to mummification, as well as fermentation mycelia in liquid culture and cultivation stromata in solid medium. Eight nucleosides and nucleobases, especially cordycepin and adenosine, were determined and analyzed with HPLC-DAD-Q-TOF-MS/MS technology. Cordycepin was detected in all forms of present O. xuefengensis strain at different contents, among which the highest content (37.1 μg/g) appeared in the stromata cultivated on solid medium. The content of adenosine in mycelia and stromata, respectively, reached 1155 μg/g and 1470 μg/g. Therefore, O. xuefengensis might be an alternative source for obtaining artificial fungus-caterpillar-larvae complex and producing cordycepin and adenosine.
- Published
- 2017