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5 results on '"Chun Tian"'

1. Diverse genomic species and evidences of symbiotic gene lateral transfer detected among the rhizobia associated withAstragalusspecies grown in the temperate regions of China

Author

Wen Feng Chen, Chun Tian Zhao, En Tao Wang, and Wen Xin Chen

Subjects
DNA, Bacterial, China, Rhizobiaceae, Gene Transfer, Horizontal, Molecular Sequence Data, N-Acetylglucosaminyltransferases, DNA, Ribosomal, Microbiology, Bradyrhizobium, Rhizobia, Bacterial Proteins, RNA, Ribosomal, 16S, Botany, Genetics, Molecular Biology, Phylogeny, Sequence Homology, Amino Acid, biology, Genetic transfer, Mesorhizobium, food and beverages, Astragalus Plant, Sequence Analysis, DNA, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Housekeeping gene, RNA, Bacterial, Sinorhizobium, bacteria, Rhizobium, Oxidoreductases
Abstract

Based on the analyses of ribosomal DNA and housekeeping genes, a total of 118 bacterial isolates obtained from 13 Astragalus species grown in the temperate region of China were identified as 19 genomic species of Mesorhizobium, Rhizobium, Sinorhizobium and Bradyrhizobium, two of them being putatively new species. Phylogenetic comparison of symbiotic genes (nodC and nifH) and housekeeping genes showed that the symbiotic genes of the Astragalus rhizobia were maintained by both vertical and horizontal transfer. The results demonstrated that the Astragalus species were very promiscuous hosts for rhizobia and that their rhizobia had very diverse genomic and symbiotic gene backgrounds.

Published
2008
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2. Different Mesorhizobium species associated with Caragana carry similar symbiotic genes and have common host ranges

Author

Wen Feng, Chen, Su Hua, Guan, Chun Tian, Zhao, Xue Rui, Yan, Chao Xin, Man, En Tao, Wang, and Wen Xin, Chen

Subjects
DNA, Bacterial, Proteome, Sequence Homology, Amino Acid, Molecular Sequence Data, Sequence Analysis, DNA, N-Acetylglucosaminyltransferases, DNA Fingerprinting, DNA, Ribosomal, Caragana, Rec A Recombinases, Bacterial Proteins, Genes, Bacterial, RNA, Ribosomal, 16S, DNA, Ribosomal Spacer, Electrophoresis, Polyacrylamide Gel, Oxidoreductases, Symbiosis, Alphaproteobacteria
Abstract

Fourteen strains representing 11 Caragana-nodulating Mesorhizobium genomic species were identified as representing Mesorhizobium amorphae, Mesorhizobium huakuii, Mesorhizobium septentrionale and groups related to Mesorhizobium plurifarium, Mesorhizobium temperatum, Mesorhizobium tianshanense and Mesorhizobium mediterraneum by sequencing of the 16S rRNA gene, 16S-23S internal transcribed spacer, partial housekeeping recA gene, and previously performed sodium dodecyl sulfate polyacrylamide gel electrophoresis of proteins and BOX-PCR fingerprinting. Despite their different taxonomic affiliation, highly similar symbiotic genes (93% similarity for nodC and91.8% similarity for nifH) were found among the Caragana strains and the three type strains for M. tianshanense, M. temperatum and M. septentrionale. Cross nodulation tests revealed that each of these 14 Caragana mesorhizobia and the three type strains mentioned above could effectively infect each of their original host plants, Caragana microphylla, Glycyrrhiza (host for M. tianshanense type strain) and Astragalus adsurgens (host for M. temperatum and M. septentrionale type strains). These results provide evidence that different Mesorhizobium species can nodulate with Caragana, and they have similar symbiotic genes (probably acquired by a phenomenon of lateral gene transfer) and common host ranges.

Published
2008

5. Diverse genomic species and evidences of symbiotic gene lateral transfer detected among the rhizobia associated with Astragalus species grown in the temperate regions of China.

Author

Zhao, Chun Tian, Wang, En Tao, Chen, Wen Feng, and Chen, Wen Xin

Subjects
ASTRAGALUS (Plants), CLADISTIC analysis, RHIZOBIUM, PHYLOGENY, SPECIES diversity, GENOMICS, GENETIC transformation, GENES, DNA
Abstract

Based on the analyses of ribosomal DNA and housekeeping genes, a total of 118 bacterial isolates obtained from 13 Astragalus species grown in the temperate region of China were identified as 19 genomic species of Mesorhizobium, Rhizobium, Sinorhizobium and Bradyrhizobium, two of them being putatively new species. Phylogenetic comparison of symbiotic genes ( nodC and nifH) and housekeeping genes showed that the symbiotic genes of the Astragalus rhizobia were maintained by both vertical and horizontal transfer. The results demonstrated that the Astragalus species were very promiscuous hosts for rhizobia and that their rhizobia had very diverse genomic and symbiotic gene backgrounds. [ABSTRACT FROM AUTHOR]

Published
2008
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