1. Isolation and characterization of a motile hydrogenotrophic methanogen from rice paddy field soil in Japan.
- Author
-
Tonouchi A
- Subjects
- Anaerobiosis, Hydrogen metabolism, Japan, Locomotion, Methanospirillum growth & development, Methanospirillum metabolism, Methanospirillum physiology, Oryza, Phylogeny, Sequence Homology, Nucleic Acid, Temperature, Methanospirillum isolation & purification, Soil Microbiology
- Abstract
A hydrogenotrophic motile methanogen was isolated from flooded Japanese paddy field soil. Anaerobic incubation of the paddy soil on H(2)-CO(2) at 20 degrees C led to the enrichment of symmetrically curved motile autofluorescent rods. The methanogenic strain TM20-1 isolated from the culture was halotolerant and utilized H(2)-CO(2), 2-propanol-CO(2), or formate as a sole methanogenic substrate. Based on the 16S rRNA gene sequence similarity (94.8%) with Methanospirillum hungateii, and on the physiological and phenotypic characteristics, TM20-1 was suggested to be a newly identified species belonging to the genus Methanospirillum. This is the first report of isolation of the genus Methanospirillum strain from a rice paddy field.
- Published
- 2002
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