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Your search keyword '"Valentina Martin"' showing total 2 results
Start Over Author "Valentina Martin" Journal fems microbiology letters
2 results on '"Valentina Martin"'

1. Expression of a cDNA encoding aToxoplasma gondiiprotein belonging to the heat-shock 90 family and analysis of its antigenicity

Author

Pablo Christian Echeverria, Mónica Nigro, Paola A. Rojas, Valentina Martin, Sergio O. Angel, Viviana Pszenny, and Eduardo A. Guarnera

Subjects
Antigenicity, DNA, Complementary, Blotting, Western, Molecular Sequence Data, Protozoan Proteins, Antibodies, Protozoan, Microbiology, Immunoglobulin G, Mice, Heat shock protein, Complementary DNA, parasitic diseases, Genetics, Animals, Humans, Amino Acid Sequence, HSP90 Heat-Shock Proteins, Molecular Biology, Mice, Inbred BALB C, Expressed sequence tag, biology, Toxoplasma gondii, biology.organism_classification, Virology, Molecular biology, Isotype, Recombinant Proteins, Mice, Inbred C57BL, Toxoplasmosis, Animal, biology.protein, Female, Antibody, Toxoplasma, Toxoplasmosis
Abstract

A cDNA clone (Tgzy85d11.r1) obtained from the Toxoplasma Expressed Sequence Tag project was chosen due to its homology with proteins of the heat shock 90 family. The cDNA encodes 137 amino acids of the C-terminal portion of the Toxoplasma Hsp90 protein (TgHsp90). Serum samples obtained from orally infected BALB/c and C57BL/6 mice showed reactivity against a recombinant TgHsp90 (rTgHsp90) after 8 weeks postinfection. Isotype analysis showed an anti-rTgHsp90 IgG2a/IgG3 response in infected BALB/c and anti-rTgHsp90 IgG1/IgG2a/IgG2b response in infected C57BL/6 mice. Serum samples from individuals chronically and putative acutely infected with T. gondii showed a similar anti-rTgHsp90 IgG response. Our work identifies TgHsp90 as a novel parasite antigen that seems to elicit a higher relation of anti-TgHsp90/anti-T. gondii IgGs during chronic infection in comparison with the acute stage.

Published
2000
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2. Characterisation of a novel interspersedToxoplasma gondiiDNA repeat with potential uses for PCR diagnosis and PCR-RFLP analysis

Author

Paola A. Rojas, Eduardo A. Guarnera, Sergio O. Angel, Viviana Pszenny, Valentina Martin, and Pablo Christian Echeverria

Subjects
Pseudogene, Molecular Sequence Data, Interspersed repeat, Biology, Polymerase Chain Reaction, Microbiology, law.invention, law, Sequence Homology, Nucleic Acid, parasitic diseases, Genetics, Animals, Amino Acid Sequence, Cloning, Molecular, Molecular Biology, Polymerase chain reaction, Repetitive Sequences, Nucleic Acid, Gel electrophoresis, Expressed sequence tag, Base Sequence, Sequence Homology, Amino Acid, Toxoplasma gondii, Sequence Analysis, DNA, DNA, Protozoan, biology.organism_classification, Molecular biology, Electrophoresis, Gel, Pulsed-Field, Genetic marker, Restriction fragment length polymorphism, Sequence Alignment, Toxoplasma, Polymorphism, Restriction Fragment Length, Toxoplasmosis
Abstract

A novel Toxoplasma gondii interspersed repeat element (TgIRE), present in most of the tachyzoite chromosomes, was characterised. Two regions on the TgIRE sequence showed high identity to two different T. gondii expressed sequence tag cDNAs of unknown function, which seems to be TgIRE pseudogenes. Two set of primers were designed, 2-2' and 2-3, that amplify products of 1.02 and 0.62 kb, respectively. T. gondii DNA from RH and Me49 strains was amplified with TgIRE 2-2' primers, and the respective 1.02 kb products were digested with several endonucleases. Different fragment patterns by gel electrophoresis were found only with MboI. Sensitivity analysis revealed that the set 2-3 was more sensitive than 2-2', detecting by gel visualisation the amount of DNA equivalent to 1 and 10 parasites, respectively.

Published
2000
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