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Your search keyword '"luminescent bacteria"' showing total 4 results
Start Over Descriptor "luminescent bacteria" Journal fems microbiology letters
4 results on '"luminescent bacteria"'

1. Bioluminescence-mediated stimulation of photoreactivation in bacteria

Author

Kozakiewicz, Jowita, Gajewska, Magdalena, Łyżeń, Robert, Czyż, Agata, and Węgrzyn, Grzegorz

Subjects
*BIOCHEMISTRY, *BIOCHEMICAL genetics, *ENTEROBACTERIACEAE, *ESCHERICHIA coli
Abstract

Abstract: Although biochemistry and genetics of light emission by cells have been investigated in detail, a biological role for bacterial luminescence has remained obscure for a long time. It was proposed recently that luminescence may stimulate DNA repair, but the specific mechanism of this phenomenon was not investigated. Moreover, experiments showing decreased survival of UV-irradiated lux mutants relative to luminescent cells were performed previously using only one bacterial species, Vibrio harveyi. Here, we demonstrate that dark mutants of various strains of naturally luminescent bacteria (Photobacterium leiognathi, Photobacterium phosphoreum and Vibrio fischeri) are more sensitive to UV irradiation than wild-type cells. Thus, this phenomenon occurs not only in V. harveyi but also in other bacterial species. Using an artificial system of luminescent Escherichia coli in combination with phr mutants (defective in photolyase functions), we found that bacterial luminescence may stimulate photoreactivation, perhaps by providing photons that are necessary for photolyase activity. [Copyright &y& Elsevier]

Published
2005
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2. Seasonal changes in luminescent intestinal microflora of the fish inhabiting the Bering and Okhotsk seas

Author

M. N. Konopleva, Svetlana A. Khrulnova, Sergey V. Bazhenov, and Ilya V. Manukhov

Subjects
Luminescence, Photobacterium phosphoreum, Oceans and Seas, Aliivibrio, Zoology, Microbiology, 03 medical and health sciences, Genetics, medicine, Animals, Psychrophile, Molecular Biology, 030304 developmental biology, 0303 health sciences, Aliivibrio logei, biology, 030306 microbiology, Photobacterium, Luminescent bacteria, Fishes, Seasonality, medicine.disease, biology.organism_classification, Gastrointestinal Microbiome, %22">Fish , Seasons, Bacteria
Abstract

Here, we present a study of luminescent intestinal microflora of the fish inhabiting Bering and Okhotsk seas in summer and winter seasons. Sampling of intestinal luminescent microflora was carried for several years, with all recovered species belonging to psychrophilic bacteria of either Aliivibrio logei or Photobacterium phosphoreum species. A seasonal change in fish intestinal luminescent microflora detected include an increase in prevalence of P. phosphoreum bacteria in summer and an increase in prevalence of A. logei bacteria in winter seasons. In fact, 90% of all luminescent bacteria isolated in winter period (January-March) were A. logei, while 88% of luminescent isolates recovered in summer period (July-September) were that of P. phosphoreum species. Seasonal changes were similar across all six sampling expeditions, three in winter and three in summer seasons, evenly spread through 2010-2018 period.

Published
2018

3. Extended-spectrum β-lactamase-producing Escherichia coli contributes to the survival of cefotaxime-susceptible E. coli under high concentrations of cefotaxime by acquisition of increased AmpC expression

Author

Ryuji Kawahara, Yoshimasa Yamamoto, Tatsuya Nakayama, and Yuko Kumeda

Subjects
0301 basic medicine, Cefotaxime, Luminescence, medicine.drug_class, 030106 microbiology, Antibiotics, Gene Expression, Microbial Sensitivity Tests, medicine.disease_cause, Microbiology, beta-Lactam Resistance, beta-Lactamases, 03 medical and health sciences, Feces, Bacterial Proteins, Oral administration, polycyclic compounds, Genetics, medicine, Escherichia coli, Animals, Cefoxitin, Molecular Biology, Escherichia coli Infections, Gel electrophoresis, Microbial Viability, Chemistry, Pseudomonas aeruginosa, Luminescent bacteria, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Anti-Bacterial Agents, Mice, Inbred C57BL, 030104 developmental biology, bacteria, medicine.drug
Abstract

Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-E) are becoming increasingly widespread in Vietnam. Antibiotics are detected in many Vietnamese foods; however, the effect of ESBL-E and antibiotic consumption on intestinal bacteria has not been studied sufficiently. Here, we investigated the effect of oral administration of ESBL-E (TB19) and cefotaxime on luminescence-emitting cefotaxime-sensitive E. coli (X14). Mice were given water containing TB19 and then received three injections of 1.0 × 108 CFU of X14 harboring a luciferase gene. The mice were administered 100 μg of cefotaxime and luminescent bacteria were monitored over 24 h, following which luminescent bacteria were isolated from mouse feces. Luminescence continued to be detected in mice administered TB19 24 h after cefotaxime ingestion. Fecal analysis revealed two types of luminescent colonies: cefoxitin-resistant E. coli (X14-R) and Pseudomonas aeruginosa. Pulse-field gel electrophoresis confirmed that X14-R was a clonal strain of X14, suggesting that X14 survived using ESBLs originating from TB19 and acquired cefoxitin resistance due to cefotaxime consumption. Moreover, in vitro analysis of X14 indicated that expression of the ampC gene was upregulated by cefotaxime. Overall, ESBL-E and cefotaxime promoted the expansion of cefoxitin-resistant E. coli in the absence of plasmid-mediated gene transfer.

Published
2017

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