1. Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations
- Author
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Tsvia Frumkin, Adi Reches, Nivin Samara, Shiri Barbash-Hazan, Joseph Hasson, Hadar Amir, Mira Malcov, Yael Kalma, Dalit Ben-Yosef, and Foad Azem
- Subjects
0301 basic medicine ,Male ,animal structures ,Embryonic Development ,Chromosomal translocation ,Fertilization in Vitro ,Biology ,Preimplantation genetic diagnosis ,Translocation, Genetic ,Andrology ,Embryo Culture Techniques ,03 medical and health sciences ,0302 clinical medicine ,Implantation failure ,Pregnancy ,Genetics ,Humans ,Embryo Implantation ,Sperm Injections, Intracytoplasmic ,Time-Lapse Imaging ,Paternal Inheritance ,Genetics (clinical) ,Preimplantation Diagnosis ,030219 obstetrics & reproductive medicine ,Pronucleus ,Obstetrics and Gynecology ,Embryo ,General Medicine ,Blastula ,Embryo Transfer ,Human genetics ,Embryo Biology ,Cell biology ,Abortion, Spontaneous ,030104 developmental biology ,Blastocyst ,Reproductive Medicine ,embryonic structures ,Female ,Developmental Biology - Abstract
PURPOSE: The purpose of the study was to compare the morphokinetic parameters of embryos carrying balanced chromosomal translocations with those carrying unbalanced chromosomal translocations using time-lapse microscopy. METHODS: The study group included 270 embryos that underwent biopsies on day 3 for preimplantation genetic diagnosis (PGD) for chromosomal translocations in our unit between 2013 and 2015. All embryos were incubated under time-lapse microscopy and evaluated for timing of developmental events up to day 5. The timing of these events was compared between balanced and unbalanced embryos, potentially viable and nonviable variants, and maternal versus paternal inheritance of the translocation. RESULTS: The PGD analysis found that 209 (77%) of the 270 biopsied embryos carried an unbalanced translocation. Embryos carrying unbalanced translocations, which are expected to lead to implantation failure or miscarriage, cleaved less synchronously and were delayed in time of cleavage to the 4-cell stage (t4) and in time of start of blastulation (tSB) compared with balanced embryos (P
- Published
- 2017