1. In utero Hematopoietic Stem Cell Transplantation: A Caprine Model for Prenatal Therapy in Inherited Metabolic Diseases
- Author
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Stacey A. Kraemer, Kathryn L. Lovell, Jodi Nichols-Torrez, Margaret Z. Jones, Kawanaa D. Carter, N. Kent Ames, Jeffrey R. Leipprandt, D.J. Sprecher, and Darush K. Rahmani
- Subjects
Male ,Embryology ,medicine.medical_treatment ,Hematopoietic stem cell transplantation ,Ultrasonography, Prenatal ,Andrology ,Metabolic Diseases ,Fetal Tissue Transplantation ,medicine ,Animals ,Radiology, Nuclear Medicine and imaging ,Hysterotomy ,Ultrasonography, Interventional ,Fetus ,business.industry ,Goats ,Peritoneal fluid ,Hematopoietic Stem Cell Transplantation ,Obstetrics and Gynecology ,General Medicine ,Transplantation ,Haematopoiesis ,In utero ,Models, Animal ,Pediatrics, Perinatology and Child Health ,Immunology ,Hepatocytes ,Feasibility Studies ,Female ,Stem cell ,business - Abstract
Objectives: We explored the feasibility and efficacy of in utero hematopoietic stem cell transplantation in the caprine animal model system with the objectives of determining procedures for transplantation and establishing methods for detecting engraftment. Methods: Male fetal liver hematopoietic stem cells were injected into female fetuses during the immunotolerant period, using either hysterotomy or ultrasound-guided injections. Results: The rate of fetal death was much lower for the ultrasound-guided injections. Donor cells were observed in the peritoneal fluid of 4 fetuses 3 days after injection, but no donor cells were detected in tissues at longer time periods. Conclusions: Ultrasound-guided injection of hematopoietic stem cells into the abdomen of a developing fetus is safe and feasible. The parameters required for successful engraftment have not yet been identified. more...
- Published
- 2000
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