1. Identification and characterization of a novel IκB-ɛ-like gene from Lamprey (Lampetra japonica) with a role in immune response.
- Author
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Su, Peng, Liu, Xin, Han, Yinglun, Zheng, Zhen, Liu, Ge, Li, Jing, and Li, Qingwei
- Subjects
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LAMPREYS , *NF-kappa B , *GENETIC transcription , *GENE expression in fishes , *VERTEBRATE genetics , *NUCLEOTIDE sequence , *AMINO acid sequence - Abstract
Abstract: Nuclear factor of kappa B (NF-κB) is a stimuli-activated transcription factor, regulates the expression of a diverse array of genes. Inhibitor of kappa B-epsilon (IκB-ɛ) is an inhibitor of NF-κB, which retains NF-κB in an inactive state in the cytoplasm. Lampreys (Lampetra japonica) belong to the lowest class of vertebrates with little information about its IκBs. We have identified a cDNA sequence IκB-ɛ-like in the lamprey and the deduced amino acid sequence of IκB-ɛ-like. It contains a conserved DSGxxS motif and six consecutive ankyrin repeats, which are necessary for signal-induced degradation of the molecule. Phylogenetic analysis indicated it had high sequence homology with IκB-ɛs from other vertebrates. FACS analysis showed that IκB-ɛ-like located in cytoplasm of leukocytes. The degradation of IκB-ɛ-like could be observed in leukocytes of L. japonica stimulated with lipopolysaccharide. These results indicate that IκB-ɛ proteins are conserved across vertebrates and the NF-κB-like signaling pathway may exist in the oldest agnatha. [Copyright &y& Elsevier]
- Published
- 2013
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