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Your search keyword '"Vazzana, M."' showing total 4 results
Start Over Author "Vazzana, M." Journal fish & shellfish immunology
4 results on '"Vazzana, M."'

4. Spontaneous cytotoxic activity of eosinophilic granule cells separated from the normal peritoneal cavity ofDicentrarchus labrax

Author

Melchiorre Cervello, Matteo Cammarata, Nicolò Parrinello, Mirella Vazzana, Vincenzo Arizza, CAMMARATA, M, VAZZANA, M, CERVELLO, M, ARIZZA, V, and PARRINELLO, N

Subjects
Cytotoxicity, Immunologic, Pathology, medicine.medical_specialty, Settore BIO/05 - Zoologia, Cell Separation, Aquatic Science, Peritoneal cavity, chemistry.chemical_compound, Lactate dehydrogenase, Centrifugation, Density Gradient, Tumor Cells, Cultured, medicine, Animals, Humans, Environmental Chemistry, Cytotoxic T cell, Cytotoxicity, Peritoneal Cavity, biology, Osmolar Concentration, Granule (cell biology), Dicentrarchus labrax, Teleostei, cytotoxicity, peritoneal cavity, eosinophilic granule cell, General Medicine, Cytotoxicity Tests, Immunologic, biology.organism_classification, Molecular biology, Eosinophils, medicine.anatomical_structure, chemistry, Cell culture, Bass, Dicentrarchus, Rabbits, Percoll
Abstract

In this study the spontaneous in vitro cytotoxic activity to tumour cell lines, (K562), by unstimulated sea bass ( Dicentrarchus labrax ) leukocytes was examined by trypan blue exclusion test and lactate dehydrogenase release assay. A high anti-tumour cell line activity of resident peritoneal leukocytes was found at an effector to target ratio (E:T) of 25:1 after incubation for 2 h at 18° C. Rabbit and sheep erythrocytes were not lysed. A low activity was displayed by head kidney and spleen cell populations whereas blood leukocytes revealed no significant activity. The effect of E:T ratio on cytotoxicity as well as microscopy observations suggested that the cytotoxic reaction required effector-target cell contact. Eosinophilic granule cells, isolated on a Percoll density gradient from a peritoneal wash, appeared to be responsible for the in vitro cytotoxic activity.

Published
2000

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