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Hepatitis B virus (HBV) infection is a well recognized occupational risk for the health care workers (HCWs). The aim of this study was to vaccinate our HCWs and to find out the facts that affect the compliance to the vaccination shedule. A total of 508 HCWs who were working in Ankara Teaching and Research Hospital during January 2002- April 2003 were enrolled into the study. Among 508 HCWs, 154 seronegative HCWs were included in the vaccination program; fourty-one (26.6%) were vaccinated with three-dose regimen, 113 (73.4%) with four-dose regimen. One hundred (64.9%) of the 154 HCWs completed the vaccination schedule successfully and 35.1% were noncompliant with the program. Being 30 years old or younger and working at jobs other than doctors or nurses were found significant factors that increase the compliance to vaccination program (p= 0.029 and p= 0.003 respectively). Working at jobs other than doctors or nurses was found significant too, by logistic regression analysis (p= 0.012). On the other hand, gender, vaccination dose number and the type of the department which they worked were not found assignificant factors influencing the compliance to the schedule (p> 0.05).