1. Interactive involvement of hippocampal cAMP/PKA and cyclooxygenase-2 signaling pathways in spatial learning in the Morris water maze
- Author
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Farzaneh Shamshirgaran, Mehdi Sanati, Sheyda Najafi, Sahar Fanoudi, Ali Akbar Nasiri, Mahmoud Hashemzaei, Kaveh Tabrizian, and Fatemeh Amelinia
- Subjects
0301 basic medicine ,Male ,Morris water navigation task ,Hippocampus ,Hippocampal formation ,Pharmacology ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Cyclic AMP ,Medicine ,Animals ,Rats, Wistar ,Maze Learning ,Protein Kinase Inhibitors ,Neuroinflammation ,Sulfonamides ,Bucladesine ,biology ,Cyclooxygenase 2 Inhibitors ,business.industry ,Isoquinolines ,Cyclic AMP-Dependent Protein Kinases ,Rats ,030104 developmental biology ,Celecoxib ,Cyclooxygenase 2 ,biology.protein ,Neurology (clinical) ,Cyclooxygenase ,Signal transduction ,business ,030217 neurology & neurosurgery ,medicine.drug ,Signal Transduction - Abstract
Introduction Accumulated evidence shows that the cAMP-PKA signaling pathway plays a key role in memory functions. Cyclooxygenase-2, a critical player in neuroinflammation, has been confirmed in the pathogenesis of neurodegenerative diseases. This study is aimed to assess the effect of the interaction of cAMP-PKA and cyclooxygenase pathways on spatial memory acquisition in animal models. Material and methods In the present study, the effects of the four-day bilateral intra-hippocampal infusions of H-89 as a protein kinase AII inhibitor (10 µM/side), celecoxib (0.1 M/side) as a selective cyclooxygenase-2 inhibitor, cele-coxib/H-89 and bucladesine (10 µM/side)/celecoxib/H-89 on spatial memory acquisition in the Morris water maze were investigated. Control animals received bilateral intra-hippocampal infusions of dimethyl sulfoxide. Rats were trained for 4 days; each day included one block of four trials. Post-training probe trial tests were performed on day five. Results A bilateral intra-hippocampal infusion of H-89 and celecoxib led to a significant impairment in spatial learning compared to the controls through a notable decrease in escape latency and traveled distance. But, combination treatment of animals with celecoxib/H-89 and bucladesine/celecoxib/H-89 could considerably reverse celecoxib and H-89-induced spatial memory acquisition impairments in the Morris water maze. Conclusions These results indicate the probable regulatory effects of cAMP/PKA and cyclooxygenase-2 signaling pathways on spatial memory acquisition in the Morris water maze.
- Published
- 2018