1. Di (2-ethylhexyl) phthalate (DEHP)-induced hepatotoxicity in quails (Coturnix japonica) via triggering nuclear xenobiotic receptors and modulating cytochrome P450 systems.
- Author
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Zhang, Ying-Zhi, Zuo, Yu-Zhu, Du, Zheng-Hai, Xia, Jun, Zhang, Cong, Wang, Hui, Li, Xue-Nan, and Li, Jin-Long
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PHTHALATE esters , *HEPATOTOXICOLOGY , *QUAILS , *XENOBIOTICS , *CYTOCHROME P-450 - Abstract
Abstract Di (2-ethylhexyl) phthalate (DEHP) is a widely distributed pollutant that is of great concern due to its negative health effects. However, whether DEHP exposure causes liver toxicity in birds remains unclear. To clarify the potential hepatotoxicity of DEHP, quails were exposed to 0, 250, 500 and 1000 mg/kg BW/day DEHP by gavage treatment for 45 days. The livers of DEHP-exposed quails showed histomorphological changes. DEHP exposure induced a significant increase in cytochrome P450 enzyme system (CYP450s) activity (including aniline-4-hydroxylase (AH), aminopyrine N-demethylase (APND), erythromycin N-demethylase (ERND) and NADPH-cytochrome C reductase (NCR)) and in the contents of total cytochrome P450 (CYP450) and cytochrome b5 (Cyt b5) in quail liver. DEHP exposure also influenced the expression of nuclear xenobiotic receptors (NXRs) and CYP450 isoforms in the liver. The results suggested that DEHP-induced hepatotoxicity in quail liver is associated with activation of the NXRs pathway responses and disruption of CYP450s homeostasis. This study will help to further elucidate DEHP exposure-induced liver toxicity in quails. Graphical abstract DEHP exposure induced hepatic toxicity. DEHP disrupted hepatic CYP450s homeostasis via affecting the transcription of CYPs. DEHP-induced toxicity was associated with activating AHR/PXR/CAR pathway responses and disrupting the CYP450s homeostasis. Image 1 Highlights • DEHP exposure induces the hepatotoxicity in quail. • DEHP triggers the hepatic AHR/PXR/CAR pathway response. • DEHP disrupts the hepatic CYP450 homeostasis. • DEHP modulates the CYPs isoforms transcription via activating the NXRs pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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