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Effects of red raspberry extract (RRE) intake on hyperlipidemia mice induced by high-fat diet (HFD) were investigated in this study. After intragastric gavage of RRE for 8 weeks, the body weight and the adipose tissue mass of mice in RRE administration groups significantly (p < 0.05) decreased compared to the group without RRE treatment. RRE treatment significantly (p < 0.05) lowered triglyceride and total cholesterol levels of hyperlipidemia mice. Pparα, Hmgcr, Ldlr, Cyp7a1, Acsl3, Pnpla2 and Pin4 were confirmed as the regulatory genes by transcriptome analysis and qRCR validation. According to KEGG pathway analysis, target genes such as Cyp7a1 and Pin4 were further regulated by the activation of PPARα resulting from RRE supplementation. Meanwhile, liver cholesterol synthesis and conversion were inhibited by the expressions of Hmgcr and Cyp7a1 genes regulated by RRE intake, and Ldlr gene was down-regulated to limit the transport of cholesterol. In addition, RRE treatment could accelerate the conversion from triglyceride to fatty acid. To conclusion, RRE intake would be a protection against diet-induced hypertriglyceridemia. Image 1 • RRE supplementation attenuated hyperlipidemia and oxidative stress in the HFD mice. • RRE intake regulated PPARa expression to further ameliorate lipid metabolism disorder. • RRE intake reduced Hmgcr expression resulting in lower cholesterol. • RRE treatment could accelerate the conversion from triglyceride to fatty acid. [ABSTRACT FROM AUTHOR]

Abstract Effects of Maillard reaction products derived from 1 to 3 kDa soybean peptides (MRPF3) on aging ICR mice were investigated. Seven animal groups were established for 5 weeks, including one normal group and six D-galactose (1000 mg kg−1/day) treated groups. Aging control was D-galactose + saline solution, and positive controls were D-galactose + ascorbic acid (Vc) (400 mg kg−1/day) and oligofructose (400 mg kg−1/day), respectively, while the test groups are D-galactose + high (800 mg kg−1/day), medium (400 mg kg−1/day) and low (200 mg kg−1/day) doses of MRPF3. Compared with the aging controls, food intake, body weights and organ indexes returned to normal after feeding with MRPF3, and the color of feces as well as the fluorescence intensity of urine increased. The content of malondialdehyde (MDA) in the liver significantly decreased with the intake of MRPF3, and the activities of SOD and GSH-Px and the total antioxidant capacity of serum significantly increased. The abundance ratio of Bacteroidetes and Firmicutes significantly decreased in MRPF3 groups, and the abundance of Lactobacillus significantly increased, while potentially pathogenic bacteria such as Porphyromonadaceae significantly decreased. Our results showed that MRPF3 might offer a potent retardation potential for aging. Graphical abstract Image 1 Highlights • Maillard reaction products derived from 1 to 3 kDa soybean peptides (MRPF3) were made. • Aging ICR mice were treated with MRPF3 at high, medium and low doses, respectively. • Physiological indexes of aging mice returned to normality after feeding with MRPF3. • Malondialdehyde content in liver decreased, antioxidant capacity of serum increased. • Pathogenic bacterial were inhibited while beneficial ones proliferated. [ABSTRACT FROM AUTHOR]