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Highlights: [•] Extract of Crataegus pinnatifida pollen (ECPP) possesses antioxidant activity. [•] Eight phenolic compounds are determined in ECPP. [•] ECPP possesses stronger ability to protect plasmid pBR322 DNA breaks in site-specific system induced by hydroxyl radicals. [•] ECPP can protect against endogenous oxidative DNA damage in mouse lymphocytes. [ABSTRACT FROM AUTHOR]

Abstract: To understand the antioxidant properties of buckwheat honeys, we investigated their antioxidant effects on hydroxyl radical-induced DNA breaks in the non-site-specific and site-specific systems, the physicochemical properties, antioxidant activities (1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical scavenging activity, chelating, and reducing power assays), total phenolic content and individual phenolic acids were also determined. Total phenolic content of buckwheat honeys ranged from 774 to 1694mg PA/kg, and p-hydroxybenzoic and p-coumaric acids proved to be the main components in buckwheat honeys. All the buckwheat honey samples possess stronger capability to protect DNA in the non-site-specific systems than in the site-specific systems from being damaged by hydroxyl radicals. In the non-site-specific and site-specific system, buckwheat honeys samples prevented OH-induced DNA breaks by 21–78% and 5–31% over control value, respectively. [Copyright &y& Elsevier]