1. Assessment of antioxidant, antitumor and pro-apoptotic effects of Salvia fruticosa Mill. subsp. thomasii (Lacaita) Brullo, Guglielmo, Pavone & Terrasi (Lamiaceae).
- Author
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Tundis, R., Iacopetta, D., Sinicropi, M.S., Bonesi, M., Leporini, M., Passalacqua, N.G., Ceramella, J., Menichini, F., and Loizzo, M.R.
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ANTIOXIDANTS , *ANTINEOPLASTIC agents , *APOPTOSIS , *SALVIA , *COLON cancer treatment , *THERAPEUTICS - Abstract
The aim of the present study was to investigate the in vitro antioxidant and antitumor effects of Salvia fruticosa Mill subsp. thomasii (Lacaita) Brullo, Guglielmo, Pavone & Terrasi (Lamiaceae). The aerial parts were extracted by maceration with methanol. This extract was partitioned with methanol and n -hexane. Luteolin, luteolin 7- O -glucoside, rutin and salvigenin were isolated from the methanol-soluble fraction. n -Hexane fraction showed viridiflorol, β-pinene, 1,8-cineole, as main components. The methanol-soluble fraction exerted antitumor activity against human breast cancer (MCF-7 and MDA-MB-231) and human colorectal carcinoma (RKO and Caco-2) cells. TUNEL test revealed that S. fruticosa subsp. thomasii leads to cells death by apoptosis, with low cytotoxic effects on non-tumoral 3T3-L1 cells. Moreover, it exerted the highest protection of lipid peroxidation and reduced the oxidative stress induced by menadione treatment in 3T3-L1 murine fibroblasts. S. fruticosa subsp. thomasii bioactivity could promote its use not only as food but also in nutraceutical/pharmaceutical industries. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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