1. Development of an LC-MS/MS method for the determination of multiple mycotoxins in human urine.
- Author
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Chen, Mobing, Liu, Wei, Xiang, Ping, Xu, Linhao, Tang, Yiling, Kong, Ran, Yang, Shuo, Yan, Hui, and Di, Bin
- Subjects
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MYCOTOXINS , *LIQUID chromatography-mass spectrometry , *URINE , *LIQUID-liquid extraction - Abstract
A sensitive liquid chromatography-tandem mass spectrometry method was developed for the determination of 17 mycotoxins in human urine. The method incorporates a two-step liquid–liquid extraction with ethyl acetate:acetonitrile (7:1), which had good extraction recovery. The LOQs of all mycotoxins ranged from 0.1 to 1 ng/mL. Intra-day accuracy ranged from 94 to 106%, and intra-day precision ranged from 1 to 12% for all mycotoxins. Inter-day accuracy and precision were 95–105% and 2–8%, respectively. The method was successfully applied to investigate the urine levels of 17 mycotoxins from 42 volunteers. Deoxynivalenol (DON, 0.97–9.88 ng/mL) was detected in 10 (24%) urine samples and zearalenone (ZEN, 0.13–1.11 ng/mL) in 2 (5%) urine samples. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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