Search

As the population ages, the pursuit of extended lifespan and improved healthspan has gained significance. This study assessed the anti-aging properties of sea cucumber peptides derived from Acaudina leucoprocta(SCP) through microbiome and metabolome analyses in Drosophila melanogaster. Supplementation with 0.2%, 0.4%, 0.6%, and 0.8% of SCP significantly boosted the survival rate and extended the mean and median lifespans in both male and female flies. Among them, 0.6% SCP treatment extended the mean and median lifespans of male flies by 21.01% and 25.48 %, which showed the most prominent lifespan-prolonging effect. Moreover, SCP enhanced the healthspan of male flies by improving their climbing ability, intestinal barrier function, and memory performance. Additionally, SCP boosted the survival rate of flies subjected to oxidative, starvation, and heat stress, with no significant effect observed under cold stress conditions. The lifespan-extension effect of SCP remained unaffected by dietary restriction, but was partially inhibited when provided with antibiotic-supplemented food. SCP modulated microbiota with increased the abundance of Lactobacillus, Rhodococcus, and Propioniciclavaand decreased Acetobacter, Achromobacter, Pedobacter, and norank_f_Saprospiraceae, which closely related with lifespan-extension effect. Furthermore, SCP induced a metabolic profile in 30-day-old flies more akin to that of 3-day-old flies, impacted 13 significantly regulated metabolites associated with amino acid, nucleotide, and carbohydrate metabolisms. These findings highlight SCP intervention as a promising anti-aging strategy.

Constipation is a widespread gastrointestinal issue. This work aimed to investigate anti-constipation capacities of 9 novel strains of Lactobacillusand Bifidobacteriumusing in vitroexperiments for initial exploration and then further confirmed through in vivomodels including both zebrafish and mice. The in vitroexperimental results revealed that L. plantarumT34 and L. paracaseiYJ10 could survive under the simulated gastrointestinal tract conditions as standard strain L. rhamnosus(LGG) did. L. paracaseiYJ10 and L. caseiLc showed the highest 22 h's aggregation rate. L. rhamnosusW1 showed the highest coaggregation rate with E. coliand with S. aureus. T34 had the largest inhibition zones against S. typhimuriumand L. Monocytogenes.The highest adhesion rates with HT-29 cells were L. caseiLc, LGG and W1. Principal component analysis (PCA) and Fuzzy synthetic evaluation (FSE) were performed to evaluate the in vitroprobiotic potential of the 9 strains comprehensively. The order of scores on in vitroprobiotic property from high to low was as follows: T34, W1, YJ10, Lc, LGG, SLT, SS, Lb, YN4 and LQSQ. The subsequent in vivoexperiment of zebrafish further confirmed T34, W1 and YJ10 have better anti-constipation ability compared to other strains. Furthermore, T34 relieve loperamide-induced constipation by promoting gut transit in the mice model. In conclusion, T34 was demonstrated to prevent constipation and may be suggested for use in food and pharmaceutical goods.