1. Declined flesh quality resulting from niacin deficiency is associated with elevated glycolysis and impaired mitochondrial homeostasis in grass carp (Ctenopharyngodon idella).
- Author
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Sun, Shun-Shi, Feng, Lin, Jiang, Wei-Dan, Liu, Yang, Ren, Hong-Mei, Jin, Xiao-Wan, Zhou, Xiao-Qiu, and Wu, Pei
- Subjects
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NIACIN , *CTENOPHARYNGODON idella , *HOMEOSTASIS , *LACTATE dehydrogenase , *GLYCOLYSIS , *PYRUVATE kinase - Abstract
[Display omitted] • Niacin deficiency decreased the growth performance of fish. • Niacin deficiency affected muscle amino acid and fatty acid compositions of fish. • Niacin deficiency exerted negative impacts on flesh physicochemical properties. • Niacin deficiency may quicken flesh pH drop by promoting glycolysis. • Niacin deficiency impaired mitochondrial homeostasis. Energy metabolism exerts profound impacts on flesh quality. Niacin can be transformed into nicotinamide adenine dinucleotide (NAD), which is indispensable to energy metabolism. To investigate whether niacin deficiency could affect energy metabolism and flesh quality, six diets with graded levels of 0.49, 9.30, 21.30, 33.30, 45.30 and 57.30 mg/kg niacin were fed to grass carp (Ctenopharyngodon idella) for 63 days. The results showed that niacin deficiency declined flesh quality by changing amino acid and fatty acid profiles, decreasing shear force, increasing cooking loss and accelerating pH decline. The accelerated pH decline might be associated with enhanced glycolysis as evident by increased hexokinase (HK), pyruvate kinase (PK) and lactic dehydrogenase (LDH) activities, and mitochondrial dysfunction as evident by destroyed mitochondrial morphology, impaired respiratory chain complex I and antioxidant ability. Based on PWG and cooking loss, the niacin requirements for sub-adult grass carp were 31.95 mg/kg and 29.66 mg/kg diet, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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