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Concern about food safety triggers demand on rapid, accurate and on-site detection of foodborne pathogens. Among various fluorescent probes for detection, carbon dots (CDs) prepared by carbonization of carbon-rich raw materials show extraordinary performance for their excellent and tailorable photoluminescence property, as well as their facilely gained specificity by surface customization and modification. CDs-based fluorescent probes play a crucial role in many pathogenic bacteria sensing systems. In addition, microfluidic technology with characteristics of portability and functional integration is expected to combine with CDs-based fluorescent probes for point-of-care testing (POCT), which can further enhance the detection property of CDs-based fluorescent probes. Here, this paper reviews CDs-based bacterial detection methods and systems, including the structural modulation of fluorescent probes and pathogenic bacteria detection mechanisms, and describes the potential of combining CDs with microfluidic technology, providing reference for the development of novel rapid detection technology for pathogenic bacteria in food., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The quality of wine can be affected by excess Cu 2+ due to the occurrence of oxidation reactions or precipitation. Therefore, it is essential to use simple and effective testing methods to ensure the Cu 2+ content in wine. In this work, we designed and synthesized a rhodamine polymer fluorescent probe (PEG-R). The water solubility of PEG-R was improved by the introduction of polyethylene glycol, which improved the performance and broadened its application in the food field. The PEG-R was characterized by high sensitivity, selectivity and fast response to Cu 2+ and was able to complete the response process within 30 s, with approximately 29-fold fluorescence enhancement of the probe after exposure to Cu 2+ , the limit of detection (LOD) was 1.295 × 10 -6 M. The probe can be used for the determination of Cu 2+ in living cells, zebrafish, white wine and food products, and it was made into practical gels and test strips., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)